Literature DB >> 181504

An improved two dimensional thin-layer chromatography system for the separation of phosphatidylglycerol and its derivatives.

B J Poorthuis, P J Yazaki, K Y Hostetler.   

Abstract

A two dimensional thin-layer chromatography system has been devised for the improved separations of phosphatidylglycerol and its derivaties, cardiolipin and bis/monoacylglyceryl)phosphate, from the other phospholipid components of tissue total lipid extracts. The system employs silica gel G plates prepared with 0.4 M boric acid. Linear recovery of added phosphatidylglycerol was found, and phosphatidylglycerol did not cochromatograph with N, N-dimethylphosphatidylethanolamine in this system. The phospholipid class composition of various rat tissues and a Morris 7777 hepatoma has been determined and compared with values from the literature.

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Year:  1976        PMID： 181504

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  55 in total

1.  Surfactant protein composition of lamellar bodies isolated from rat lung.

Authors:  M A Oosterlaken-Dijksterhuis; M van Eijk; B L van Buel; L M van Golde; H P Haagsman
Journal:  Biochem J       Date:  1991-02-15       Impact factor: 3.857

2.  Cytochrome c oxidase is required for the assembly/stability of respiratory complex I in mouse fibroblasts.

Authors:  Francisca Diaz; Hirokazu Fukui; Sofia Garcia; Carlos T Moraes
Journal:  Mol Cell Biol       Date:  2006-07       Impact factor: 4.272

3.  Phosphatidylserine as a determinant for the tissue distribution of weakly basic drugs in rats.

Authors:  N Yata; T Toyoda; T Murakami; A Nishiura; Y Higashi
Journal:  Pharm Res       Date:  1990-10       Impact factor: 4.200

4.  Isolation of alveolar type II cells by centrifugal elutriation.

Authors:  R D Greenleaf; R J Mason; M C Williams
Journal:  In Vitro       Date:  1979-09

5.  N-Acetylsphingosine stimulates phosphatidylglycerolphosphate synthase activity in H9c2 cardiac cells.

Authors:  F Y Xu; S L Kelly; G M Hatch
Journal:  Biochem J       Date:  1999-02-01       Impact factor: 3.857

Review 6.  Regulation of cardiolipin biosynthesis in the heart.

Authors:  G M Hatch
Journal:  Mol Cell Biochem       Date:  1996-06-21       Impact factor: 3.396

7.  Characterization of phospholipids accumulated in pulmonary-surfactant compartments of rats intratracheally exposed to silica.

Authors:  H Adachi; H Hayashi; H Sato; K Dempo; T Akino
Journal:  Biochem J       Date:  1989-09-15       Impact factor: 3.857

8.  High performance liquid chromatographic separation of diacylglycerol acetates to quantitate disaturated species of lung phosphatidylcholine.

Authors:  K Itoh; A Suzuki; Y Kuroki; T Akino
Journal:  Lipids       Date:  1985-09       Impact factor: 1.880

9.  Decrease in cardiac phosphatidylglycerol in streptozotocin-induced diabetic rats does not affect cardiolipin biosynthesis: evidence for distinct pools of phosphatidylglycerol in the heart.

Authors:  G M Hatch; S G Cao; A Angel
Journal:  Biochem J       Date:  1995-03-15       Impact factor: 3.857

10.  On the mechanism of the phospholipase C-mediated attenuation of cardiolipin biosynthesis in H9c2 cardiac myoblast cells.

Authors:  F Y Xu; S L Kelly; W A Taylor; G M Hatch
Journal:  Mol Cell Biochem       Date:  1998-11       Impact factor: 3.396
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