Temperature activation of certain respiratory enzymes of stenothermophilic bacteria.

The results of this study of the effect of temperature on the respiratory mechanism of five stenothermophilic bacteria may be summarized as follows:- 1. The respiratory mechanism and its various components of the stenothermophilic bacteria were found to function at temperatures below the minimum temperature for growth of these organisms. In every case the rates of the individual reactions involved in the respiratory chain increased exponentially with temperature until the temperature at which inactivation became apparent was reached. 2. The mean activation energies, calculated from the "best" value for the slope of the straight lines resulting from a plot of log rate against the reciprocal of the absolute temperature were: Dehydrogenases: 28,000 to 28,500 calories per gram molecule. Glucose, fructose, galactose, mannose, xylose, arabinose, maltose, lactose, sucrose, glycine, beta-alanine, monosodium glutamate, (asparagine). 19,500 to 20,500 calories per gram molecule. Ethyl alcohol, succinate, pyruvate, lactate, acetate. 19,500 to 20,500 calories per gram molecule. Ethyl alcohol, succinate, pyruvate, lactate, acetate. 15,000 calories per gram molecule. Formate. Cytochrome oxidase and cytochrome b and c (substrate: p-phenylenediamine): 16,800 calories per gram molecule. Cytochrome oxidase and cytochrome c (substrate: hydroquinone): 20,200 calories per gram molecule. Catalase: 4,100 calories per gram molecule. Complete aerobic respiratory system (plus added glucose): 29,500 calories per gram molecule. 3. The identity of the energies of activation of the respiratory system and its enzymic components at temperatures above and below the minimum temperature for growth of the stenothermophilic bacteria was demonstrated. 4. An attempt has been made to indicate a relationship between the nature of the substrate and the activation energy by grouping substrates on the basis of common micro values obtained for their dehydrogenation by resting cell preparations of stenothermophilic bacteria. The dehydrogenation reactions have been found to be the rate-controlling reactions in the aerobic respiratory system of these bacteria.