INTRODUCTION: The lesser pathogenicity of HIV-2 relative to HIV-1 is generally attributed to its slower replication. To compare the amounts of total HIV DNA during human HIV-1 and HIV-2 infection, we developed a quantitative real-time PCR method with a unique external quantification standard based on a single plasmid harboring both the HIV-1 and the HIV-2 LTR. METHODS: Viral DNA load was compared between 40 HIV-1-infected and 42 HIV-2-infected antiretroviral-naive patients. RESULTS: The difference between HIV-1 and HIV-2 proviral DNA load was highly significant in patients with CD4 cell counts > 500 cells/microl [HIV-1: n = 14; median, 2.5; interquartile range (IQR), 2.1-2.7; HIV-2: n = 22, median, 1.6; IQR, 1.0-2.0] and in patients with CD4 cell counts between 300 cells/microl and 500 cells/microl (HIV-1: n = 12; median, 2.7; IQR, 2.3-2.8; HIV-2: n = 11; median, 2.0; IQR, 1.0-2.4). Too few HIV-2-infected patients had CD4 cell counts < 300 cells/microl to detect a significant difference but DNA values were again lower in HIV-2-infected patients (HIV-1: n = 14; median, 2.9; IQR, 2.2-3.2; HIV-2: n = 9; median, 2.7; IQR, 2.2-3.3). CONCLUSIONS: These differences are in line with the natural histories of the two infections and show that HIV-2 infection is a valid model for studying the pathophysiology of HIV infection in general.
INTRODUCTION: The lesser pathogenicity of HIV-2 relative to HIV-1 is generally attributed to its slower replication. To compare the amounts of total HIV DNA during humanHIV-1 and HIV-2 infection, we developed a quantitative real-time PCR method with a unique external quantification standard based on a single plasmid harboring both the HIV-1 and the HIV-2 LTR. METHODS: Viral DNA load was compared between 40 HIV-1-infected and 42 HIV-2-infected antiretroviral-naive patients. RESULTS: The difference between HIV-1 and HIV-2 proviral DNA load was highly significant in patients with CD4 cell counts > 500 cells/microl [HIV-1: n = 14; median, 2.5; interquartile range (IQR), 2.1-2.7; HIV-2: n = 22, median, 1.6; IQR, 1.0-2.0] and in patients with CD4 cell counts between 300 cells/microl and 500 cells/microl (HIV-1: n = 12; median, 2.7; IQR, 2.3-2.8; HIV-2: n = 11; median, 2.0; IQR, 1.0-2.4). Too few HIV-2-infectedpatients had CD4 cell counts < 300 cells/microl to detect a significant difference but DNA values were again lower in HIV-2-infectedpatients (HIV-1: n = 14; median, 2.9; IQR, 2.2-3.2; HIV-2: n = 9; median, 2.7; IQR, 2.2-3.3). CONCLUSIONS: These differences are in line with the natural histories of the two infections and show that HIV-2 infection is a valid model for studying the pathophysiology of HIV infection in general.
Authors: Ernesto Marin-Flamand; Diana Michele Araiza-Hernandez; Alejandro Vargas-Ruiz; Ignacio Carlos Rangel-Rodríguez; Lilia A González-Tapia; Hugo Ramírez-Álvarez; Ruperto Javier Hernández-Balderas; Lucía Angélica García-Camacho Journal: Can J Vet Res Date: 2022-10 Impact factor: 0.897
Authors: Gülşen Özkaya Şahin; Fredrik Månsson; Angelica A Palm; Elzbieta Vincic; Zacarias da Silva; Patrik Medstrand; Hans Norrgren; Eva Maria Fenyö; Marianne Jansson Journal: AIDS Res Hum Retroviruses Date: 2012-11-21 Impact factor: 2.205
Authors: Michael D Lu; Sushama Telwatte; Nitasha Kumar; Fernanda Ferreira; Holly Anne Martin; Gayatri Nikhila Kadiyala; Adam Wedrychowski; Sara Moron-Lopez; Tsui-Hua Chen; Erin A Goecker; Robert W Coombs; Chuanyi M Lu; Joseph K Wong; Athe Tsibris; Steven A Yukl Journal: PLoS One Date: 2022-04-27 Impact factor: 3.752