Literature DB >> 18097102

Preliminary X-ray crystallographic analysis of SMU.573, a putative sugar kinase from Streptococcus mutans.

Yan-Feng Zhou1, Lan-Fen Li, Cheng Yang, Yu-He Liang, Xiao-Dong Su.   

Abstract

SMU.573 from Streptococcus mutans is a structurally and functionally uncharacterized protein that was selected for structural biology studies. Native and SeMet-labelled proteins were expressed with an N-His tag in Escherichia coli BL21 (DE3) and purified by Ni2+-chelating and size-exclusion chromatography. Crystals of the SeMet-labelled protein were obtained by the hanging-drop vapour-diffusion method and a 2.5 A resolution diffraction data set was collected using an in-house chromium radiation source. The crystals belong to space group I4, with unit-cell parameters a = b = 96.53, c = 56.26 A, alpha = beta = gamma = 90 degrees.

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Year:  2007        PMID： 18097102      PMCID： PMC2373987          DOI： 10.1107/S1744309107065645

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

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5.  Solvent content of protein crystals.

Authors:  B W Matthews
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Review 6.  Role of Streptococcus mutans in human dental decay.

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8.  Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.

Authors:  N Campobasso; I I Mathews; T P Begley; S E Ealick
Journal:  Biochemistry       Date:  2000-07-11       Impact factor: 3.162

9.  Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8 A resolution: insights into a new family of kinase structures.

Authors:  J A Sigrell; A D Cameron; T A Jones; S L Mowbray
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10.  Structure of Bacillus subtilis YXKO--a member of the UPF0031 family and a putative kinase.

Authors:  R-g Zhang; J Grembecka; E Vinokour; F Collart; I Dementieva; W Minor; A Joachimiak
Journal:  J Struct Biol       Date:  2002-09       Impact factor: 2.867
  10 in total

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