| Literature DB >> 18096892 |
J Zhang1, J Zhu, P Valverde, L Li, S Pageau, Q Tu, R Nishimura, T Yoneda, P Yang, W Zheng, W Ma, J Chen.
Abstract
Dlx5 plays an important role in the embryonic development of mineralized tissues. We hypothesized that Dlx5 also functions in regulating post-natal bone formation in mice. To prove this hypothesis, we infected 5-day-old bone sialoprotein (BSP)/avian retroviral receptor gene (TVA) transgenic mice with replication-competent retroviral vectors expressing wild-type Dlx5 (RCAS-Dlx5WT) and mutated Dlx5 at arginine (R) 31 of its homeodomain (RCAS-Dlx5RH). Immunohistochemistry indicated that RCAS-Dlx5WT increased BSP and osteopontin (OPN) expression, whereas it decreased that of osteocalcin (OC). RCAS-Dlx5RH mediated opposite effects. Semi-quantitative RT-PCR confirmed these results. Ex vivo overexpression of RCAS-Dlx5WT in BSP/TVA calvarial cells promoted, whereas that of RCAS-Dlx5RH inhibited, mineralized nodule formation as compared with that in control cells. Our results suggest that Dlx5 promotes expression of early markers of osteogenic differentiation and increases mineralization post-natally.Entities:
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Year: 2008 PMID： 18096892 PMCID： PMC2833013 DOI： 10.1177/154405910808700107
Source DB: PubMed Journal: J Dent Res ISSN： 0022-0345 Impact factor: 6.116