Literature DB >> 1809325

Automated kinetic assay of beta-galactosidase activity.

D N Arvidson1, P Youderian, T D Schneider, G D Stormo.   

Abstract

An automated kinetic assay for beta-galactosidase activity in Escherichia coli was developed to permit the measurement of many independent samples simultaneously. Bacteria are grown, lysed from without (by adsorption of a high multiplicity of bacteriophage T4) and assayed in microtiter plates with 96 wells. Absorbance data are collected and analyzed by computer. The growth and lysis procedure, apparatus and software used in this assay can be used for other spectrophotometric enzyme assays.

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Year:  1991        PMID： 1809325

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  5 in total

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Journal:  Nucleic Acids Res       Date:  1994-04-11       Impact factor: 16.971

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Authors:  Jeffrey R Bernstein; Thomas Bulter; James C Liao
Journal:  Metab Eng       Date:  2008-02-13       Impact factor: 9.783

5.  Single-step method for β-galactosidase assays in Escherichia coli using a 96-well microplate reader.

Authors:  Jorrit Schaefer; Goran Jovanovic; Ioly Kotta-Loizou; Martin Buck
Journal:  Anal Biochem       Date:  2016-03-29       Impact factor: 3.365
  5 in total

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