Z Jiao1, W Wang, R Jia, J Li, H You, L Chen, Y Wang. 1. Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, China. jiaozhijun@ujs.edu.cn
Abstract
OBJECTIVE: To explore the presence and characteristics of FoxP3-expressing CD4+CD25+ regulatory T cells in synovial fluid (SF) of patients with active rheumatoid arthritis (RA). METHODS: The frequency and chemokine receptors expression profile of FoxP3-expressing CD4+CD25+ regulatory T cells in SF and peripheral blood (PB) from RA patients and PB from healthy controls were investigated by flow cytometry using three- or four-colour intracellular staining. RESULTS: The frequency of CD4+CD25+ FoxP3+ T cells was increased significantly in SF compared with paired PB from RA patients and PB from healthy controls (p<0.05). However, the frequency in PB from RA patients was significantly lower than in PB from healthy controls (p<0.05). Notably, CD4+CD25+FoxP3+ T cells in SF expressed increased levels of inflammation-related trafficking chemokine receptors, such as CCR4, CCR5, and CXCR4. CONCLUSION: There is an accumulation of FoxP3-expressing regulatory T cells in RA SF, and such recruitment may be dependent on the distinct chemokine receptors expressed on regulatory T cells.
OBJECTIVE: To explore the presence and characteristics of FoxP3-expressing CD4+CD25+ regulatory T cells in synovial fluid (SF) of patients with active rheumatoid arthritis (RA). METHODS: The frequency and chemokine receptors expression profile of FoxP3-expressing CD4+CD25+ regulatory T cells in SF and peripheral blood (PB) from RApatients and PB from healthy controls were investigated by flow cytometry using three- or four-colour intracellular staining. RESULTS: The frequency of CD4+CD25+ FoxP3+ T cells was increased significantly in SF compared with paired PB from RApatients and PB from healthy controls (p<0.05). However, the frequency in PB from RApatients was significantly lower than in PB from healthy controls (p<0.05). Notably, CD4+CD25+FoxP3+ T cells in SF expressed increased levels of inflammation-related trafficking chemokine receptors, such as CCR4, CCR5, and CXCR4. CONCLUSION: There is an accumulation of FoxP3-expressing regulatory T cells in RA SF, and such recruitment may be dependent on the distinct chemokine receptors expressed on regulatory T cells.
Authors: Paul A Beavis; Bernard Gregory; Patricia Green; Adam P Cribbs; Alan Kennedy; Parisa Amjadi; Andrew C Palfreeman; Marc Feldmann; Fionula M Brennan Journal: Proc Natl Acad Sci U S A Date: 2011-09-16 Impact factor: 11.205
Authors: Ariana Barberá; Noraylis Lorenzo; Peter van Kooten; Joel van Roon; Wilco de Jager; Dinorah Prada; Jorge Gómez; Gabriel Padrón; Willem van Eden; Femke Broere; María Del Carmen Domínguez Journal: Cell Stress Chaperones Date: 2016-05-30 Impact factor: 3.667