AIM: To use ergosterol assay as a rapid tool to assess the extent of fungal contamination in grains and feeds. METHODS AND RESULTS: Ergosterol was extracted from moulds and quantified using a modified method based on Seitz et al. (1977). A good correlation coefficient of 0.9998 was obtained for ergosterol standards and a strong correlation (R(2) = 0 x 9645) was established between the ergosterol content and the growth of Aspergillus niger ATCC 24126. This shows that this assay can be used to estimate fungal growth. The ergosterol contents and amount of carbon dioxide produced in both Control and Myco CURB (brand) liquid-treated corns were measured over 36 days. Ergosterol contents of pelleted pig feeds were also determined. In both experiments, the levels of ergosterol in the control samples were significantly higher than those of the mould-inhibitor-treated samples. CONCLUSIONS: A strong positive correlation (Spearman, Rs = 0 x 7241) was observed between the ergosterol content and the degree of fungal growth. Hence, ergosterol assay can be used as a rapid tool to assess the microbiological status of grains and feeds. SIGNIFICANCE AND IMPACT OF THE STUDY: This study affirms that ergosterol assay is a rapid and accurate tool that can be used for the assessment of the feed quality.
AIM: To use ergosterol assay as a rapid tool to assess the extent of fungal contamination in grains and feeds. METHODS AND RESULTS:Ergosterol was extracted from moulds and quantified using a modified method based on Seitz et al. (1977). A good correlation coefficient of 0.9998 was obtained for ergosterol standards and a strong correlation (R(2) = 0 x 9645) was established between the ergosterol content and the growth of Aspergillus niger ATCC 24126. This shows that this assay can be used to estimate fungal growth. The ergosterol contents and amount of carbon dioxide produced in both Control and Myco CURB (brand) liquid-treated corns were measured over 36 days. Ergosterol contents of pelleted pig feeds were also determined. In both experiments, the levels of ergosterol in the control samples were significantly higher than those of the mould-inhibitor-treated samples. CONCLUSIONS: A strong positive correlation (Spearman, Rs = 0 x 7241) was observed between the ergosterol content and the degree of fungal growth. Hence, ergosterol assay can be used as a rapid tool to assess the microbiological status of grains and feeds. SIGNIFICANCE AND IMPACT OF THE STUDY: This study affirms that ergosterol assay is a rapid and accurate tool that can be used for the assessment of the feed quality.
Authors: Robert Rusinek; Henryk Jeleń; Urszula Malaga-Toboła; Marek Molenda; Marek Gancarz Journal: Sensors (Basel) Date: 2020-06-01 Impact factor: 3.576
Authors: Jolanda M van Munster; Paul Daly; Martin J Blythe; Roger Ibbett; Matt Kokolski; Sanyasi Gaddipati; Erika Lindquist; Vasanth R Singan; Kerrie W Barry; Anna Lipzen; Chew Yee Ngan; Christopher J Petzold; Leanne Jade G Chan; Mikko Arvas; Roxane Raulo; Steven T Pullan; Stéphane Delmas; Igor V Grigoriev; Gregory A Tucker; Blake A Simmons; David B Archer Journal: Biotechnol Biofuels Date: 2020-04-13 Impact factor: 6.040
Authors: Elena Maria Colombo; Andrea Kunova; Claudio Gardana; Cristina Pizzatti; Paolo Simonetti; Paolo Cortesi; Marco Saracchi; Matias Pasquali Journal: Toxins (Basel) Date: 2020-08-31 Impact factor: 4.546