Literature DB >> 18083826

Effect of hydroxyurea on procyclic Trypanosoma brucei: an unconventional mechanism for achieving synchronous growth.

Arnab Roy Chowdhury1, Zhixing Zhao, Paul T Englund.   

Abstract

Procyclic Trypanosoma brucei cells were synchronized with 0.2 mM hydroxyurea. The cells did not arrest at the G(1)/S boundary but proceeded through one round of replication and arrested near the end of S phase. The mitochondrial genome (kinetoplast DNA network) replicated, forming two progeny networks, but the repair of minicircle gaps was inhibited.

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Year:  2007        PMID： 18083826      PMCID： PMC2238168          DOI： 10.1128/EC.00369-07

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN： 1535-9786


  20 in total

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Authors:  P K Davis; A Ho; S F Dowdy
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2.  Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control.

Authors:  M Soto; L Quijada; C Alonso; J M Requena
Journal:  Biochem J       Date:  2000-02-15       Impact factor: 3.857

3.  Multiple mitochondrial DNA polymerases in Trypanosoma brucei.

Authors:  Michele M Klingbeil; Shawn A Motyka; Paul T Englund
Journal:  Mol Cell       Date:  2002-07       Impact factor: 17.970

4.  Synchronization of Leishmania tarentolae by hydroxyurea.

Authors:  L Simpson; P Braly
Journal:  J Protozool       Date:  1970-11

5.  Ribonucleotide reductase--a radical enzyme.

Authors:  P Reichard; A Ehrenberg
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6.  Effects of hydroxyurea on Crithidia fasciculata.

Authors:  W B Cosgrove; M J Skeen; S L Hajduk
Journal:  J Protozool       Date:  1979-11

7.  Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters.

Authors:  Z Wang; J C Morris; M E Drew; P T Englund
Journal:  J Biol Chem       Date:  2000-12-22       Impact factor: 5.157

8.  Hydroxyurea: effect on growth, structure, and [3H]thymidine uptake of Trypanosoma brucei procyclic culture forms.

Authors:  R Brun
Journal:  J Protozool       Date:  1980-02

9.  Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes.

Authors:  Tina T Saxowsky; Gunjan Choudhary; Michele M Klingbeil; Paul T Englund
Journal:  J Biol Chem       Date:  2003-09-08       Impact factor: 5.157

10.  The rotational dynamics of kinetoplast DNA replication.

Authors:  Yanan Liu; Paul T Englund
Journal:  Mol Microbiol       Date:  2007-05       Impact factor: 3.501
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  27 in total

1.  Dynamic localization of Trypanosoma brucei mitochondrial DNA polymerase ID.

Authors:  Jeniffer Concepción-Acevedo; Juemin Luo; Michele M Klingbeil
Journal:  Eukaryot Cell       Date:  2012-01-27

2.  A mitochondrial DNA primase is essential for cell growth and kinetoplast DNA replication in Trypanosoma brucei.

Authors:  Jane C Hines; Dan S Ray
Journal:  Mol Cell Biol       Date:  2010-01-11       Impact factor: 4.272

3.  Trypanosoma brucei EIF4E2 cap-binding protein binds a homolog of the histone-mRNA stem-loop-binding protein.

Authors:  Eden R Freire; Danielle M N Moura; Maria J R Bezerra; Camila C Xavier; Mariana C Morais-Sobral; Ajay A Vashisht; Antonio M Rezende; James A Wohlschlegel; Nancy R Sturm; Osvaldo P de Melo Neto; David A Campbell
Journal:  Curr Genet       Date:  2017-12-29       Impact factor: 3.886

4.  Polo-like kinase guides cytokinesis in Trypanosoma brucei through an indirect means.

Authors:  Zhi Li; Takashi Umeyama; Ziyin Li; Ching C Wang
Journal:  Eukaryot Cell       Date:  2010-03-12

5.  Replication of the ERES:Golgi junction in bloodstream-form African trypanosomes.

Authors:  James D Bangs
Journal:  Mol Microbiol       Date:  2011-11-10       Impact factor: 3.501

6.  The Aurora Kinase in Trypanosoma brucei plays distinctive roles in metaphase-anaphase transition and cytokinetic initiation.

Authors:  Ziyin Li; Takashi Umeyama; C C Wang
Journal:  PLoS Pathog       Date:  2009-09-11       Impact factor: 6.823

7.  Polo-like kinase is expressed in S/G2/M phase and associated with the flagellum attachment zone in both procyclic and bloodstream forms of Trypanosoma brucei.

Authors:  Takashi Umeyama; Ching C Wang
Journal:  Eukaryot Cell       Date:  2008-07-11

8.  Cell-cycle synchronisation of bloodstream forms of Trypanosoma brucei using Vybrant DyeCycle Violet-based sorting.

Authors:  Sarah Kabani; Martin Waterfall; Keith R Matthews
Journal:  Mol Biochem Parasitol       Date:  2009-09-01       Impact factor: 1.759

9.  Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle.

Authors:  Stuart K Archer; Van-Duc Luu; Rafael A de Queiroz; Stefanie Brems; Christine Clayton
Journal:  PLoS Pathog       Date:  2009-08-28       Impact factor: 6.823

10.  Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei.

Authors:  Glynn R Forsythe; Richard McCulloch; Tansy C Hammarton
Journal:  Mol Biochem Parasitol       Date:  2008-12-27       Impact factor: 1.759
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