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Literature DB >> 18083803

Tn5 synaptic complex formation: role of transposase residue W450.

Richard J Gradman¹, William S Reznikoff.

Abstract

A series of Tn5 transposases (Tnp's) with mutations at the conserved amino acid position W450, which was structurally predicted to be important for synapsis, have been generated and characterized. This study demonstrates that W450 is involved in hydrophobic (and possibly aromatic) contacts within the Tnp monomer that negatively regulate synaptic complex formation.

Entities: Chemical

Mesh：

Substances：

Year: 2007 PMID： 18083803 PMCID： PMC2238198 DOI： 10.1128/JB.01488-07

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

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References

17 in total

Review 1. Comparative architecture of transposase and integrase complexes.

Authors: P A Rice; T A Baker
Journal: Nat Struct Biol Date: 2001-04

2. The C-terminal alpha helix of Tn5 transposase is required for synaptic complex formation.

Authors: M Steiniger-White; W S Reznikoff
Journal: J Biol Chem Date: 2000-07-28 Impact factor: 5.157

3. IS911 transposition is regulated by protein-protein interactions via a leucine zipper motif.

Authors: L Haren; C Normand; P Polard; R Alazard; M Chandler
Journal: J Mol Biol Date: 2000-02-25 Impact factor: 5.469

4. Characterization of a Tn5 pre-cleavage synaptic complex.

Authors: A Bhasin; I Y Goryshin; M Steiniger-White; D York; W S Reznikoff
Journal: J Mol Biol Date: 2000-09-08 Impact factor: 5.469

5. Phage Mu transposase: deletion of the carboxy-terminal end does not abolish DNA-binding activity.

Authors: M Betermier; R Alazard; F Ragueh; E Roulet; A Toussaint; M Chandler
Journal: Mol Gen Genet Date: 1987-11

6. Mutation of Tn5 transposase beta-loop residues affects all steps of Tn5 transposition: the role of conformational changes in Tn5 transposition.

Authors: Mindy Steiniger; Jeremy Metzler; William S Reznikoff
Journal: Biochemistry Date: 2006-12-05 Impact factor: 3.162

Tn5 synaptic complex formation: role of transposase residue W450.

Review 1. Comparative architecture of transposase and integrase complexes.

2. The C-terminal alpha helix of Tn5 transposase is required for synaptic complex formation.

3. IS911 transposition is regulated by protein-protein interactions via a leucine zipper motif.

4. Characterization of a Tn5 pre-cleavage synaptic complex.

5. Phage Mu transposase: deletion of the carboxy-terminal end does not abolish DNA-binding activity.

6. Mutation of Tn5 transposase beta-loop residues affects all steps of Tn5 transposition: the role of conformational changes in Tn5 transposition.

7. Purification and biochemical analyses of a monomeric form of Tn5 transposase.

8. Characterization of the Tn5 transposase and inhibitor proteins: a model for the inhibition of transposition.

9. Evidence that the cis preference of the Tn5 transposase is caused by nonproductive multimerization.

10. Use of a Tn5 derivative that creates lacZ translational fusions to obtain a transposition mutant.