Literature DB >> 18083186

Application of nine-color flow cytometry for detailed studies of the phenotypic complexity and functional heterogeneity of human lymphocyte subsets.

Veronica D Gonzalez1, Niklas K Björkström, Karl-Johan Malmberg, Markus Moll, Carlotta Kuylenstierna, Jakob Michaëlsson, Hans-Gustaf Ljunggren, Johan K Sandberg.   

Abstract

Innate and adaptive cellular immunity is initiated, directed and regulated by a vast array of cell surface receptors. Attempts to harness the cellular immune system in translational settings such as immunotherapy and vaccine development require tools to accurately describe and isolate lymphocytes with specific characteristics. One such tool, flow cytometry, is undergoing a revolution in instrumentation and reagents, providing opportunities for high resolution phenotypic and functional analysis of lymphocytes. Here, we demonstrate how nine-color flow cytometry can be adapted, optimized and applied to investigate the phenotypic complexity and functional heterogeneity of human lymphocyte subsets. We provide examples of studies of adaptive T cell responses against viruses, as well as the assessment of CD1d-restricted NKT cells and NK cells. We discuss the importance of this technology for detailed investigations of lymphocyte subsets in studies of infectious diseases and cancer.

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Year:  2007        PMID： 18083186      PMCID： PMC2268636          DOI： 10.1016/j.jim.2007.10.020

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  38 in total

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3.  Semiconductor nanocrystals as fluorescent biological labels.

Authors:  M Bruchez; M Moronne; P Gin; S Weiss; A P Alivisatos
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4.  HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C.

Authors:  V M Braud; D S Allan; C A O'Callaghan; K Söderström; A D'Andrea; G S Ogg; S Lazetic; N T Young; J I Bell; J H Phillips; L L Lanier; A J McMichael
Journal:  Nature       Date:  1998-02-19       Impact factor: 49.962

Review 5.  Natural killer cells in antiviral defense: function and regulation by innate cytokines.

Authors:  C A Biron; K B Nguyen; G C Pien; L P Cousens; T P Salazar-Mather
Journal:  Annu Rev Immunol       Date:  1999       Impact factor: 28.527

6.  Phenotypic analysis of antigen-specific T lymphocytes.

Authors:  J D Altman; P A Moss; P J Goulder; D H Barouch; M G McHeyzer-Williams; J I Bell; A J McMichael; M M Davis
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7.  Distinct homeostatic requirements of CD4+ and CD4- subsets of Valpha24-invariant natural killer T cells in humans.

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Review 8.  The immunoregulatory role of CD1d-restricted natural killer T cells in disease.

Authors:  Hans J J van der Vliet; Johan W Molling; B Mary E von Blomberg; Nobusuke Nishi; Wendy Kölgen; Alfons J M van den Eertwegh; Herbert M Pinedo; Giuseppe Giaccone; Rik J Scheper
Journal:  Clin Immunol       Date:  2004-07       Impact factor: 3.969

9.  Expansion of CD7(low) and CD7(negative) CD8 T-cell effector subsets in HIV-1 infection: correlation with antigenic load and reversion by antiretroviral treatment.

Authors:  Einar M Aandahl; Máire F Quigley; Walter J Moretto; Markus Moll; Veronica D Gonzalez; Anders Sönnerborg; Stefan Lindbäck; Frederick M Hecht; Steven G Deeks; Michael G Rosenberg; Douglas F Nixon; Johan K Sandberg
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10.  Phenotypic and functional separation of memory and effector human CD8+ T cells.

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Journal:  J Exp Med       Date:  1997-11-03       Impact factor: 14.307
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  11 in total

1.  Distinct infiltration of neutrophils in lesion shoulders in ApoE-/- mice.

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Journal:  Am J Pathol       Date:  2010-05-14       Impact factor: 4.307

2.  NK cells expressing inhibitory KIR for non-self-ligands remain tolerant in HLA-matched sibling stem cell transplantation.

Authors:  Andreas T Björklund; Marie Schaffer; Cyril Fauriat; Olle Ringdén; Mats Remberger; Christina Hammarstedt; A John Barrett; Per Ljungman; Hans-Gustaf Ljunggren; Karl-Johan Malmberg
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3.  Regulation of human NK-cell cytokine and chemokine production by target cell recognition.

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Review 4.  Understanding health and disease with multidimensional single-cell methods.

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5.  Human immunodeficiency virus type 1 infection is associated with increased NK cell polyfunctionality and higher levels of KIR3DL1+ NK cells in ugandans carrying the HLA-B Bw4 motif.

Authors:  Michael A Eller; Rebecca N Koehler; Gustavo H Kijak; Leigh Anne Eller; David Guwatudde; Mary A Marovich; Nelson L Michael; Mark S de Souza; Fred Wabwire-Mangen; Merlin L Robb; Jeffrey R Currier; Johan K Sandberg
Journal:  J Virol       Date:  2011-03-16       Impact factor: 5.103

6.  NKG2D performs two functions in invariant NKT cells: direct TCR-independent activation of NK-like cytolysis and co-stimulation of activation by CD1d.

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7.  High levels of chronic immune activation in the T-cell compartments of patients coinfected with hepatitis C virus and human immunodeficiency virus type 1 and on highly active antiretroviral therapy are reverted by alpha interferon and ribavirin treatment.

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8.  Severe functional impairment and elevated PD-1 expression in CD1d-restricted NKT cells retained during chronic HIV-1 infection.

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9.  Functional analysis of human NK cells by flow cytometry.

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10.  Reduction of the HIV-1 reservoir in resting CD4+ T-lymphocytes by high dosage intravenous immunoglobulin treatment: a proof-of-concept study.

Authors:  Annica Lindkvist; Arvid Edén; Melissa M Norström; Veronica D Gonzalez; Staffan Nilsson; Bo Svennerholm; Annika C Karlsson; Johan K Sandberg; Anders Sönnerborg; Magnus Gisslén
Journal:  AIDS Res Ther       Date:  2009-07-01       Impact factor: 2.250
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