| Literature DB >> 18078808 |
Congsheng Cheng1, Jie Dong, Lihong Yao, Aijun Chen, Runqing Jia, Lifang Huan, Jianqiang Guo, Yuelong Shu, Zhiqing Zhang.
Abstract
New therapeutics are urgently needed for the treatment of pandemic influenza caused by H5N1 influenza virus mutants. Aptamer was a promising candidate for treatment and prophylaxis of influenza virus infections. In this study, systemic evolution of ligands through exponential enrichment (SELEX) was used to screen DNA aptamers targeted to recombinant HA1 proteins of the H5N1 influenza virus. After 11 rounds of selection, DNA aptamers that bind to the HA1 protein were isolated and shown to have different binding capacities. Among them, aptamer 10 had the strongest binding to the HA1 protein, and had an inhibitory effect on H5N1 influenza virus, as shown by the hemagglutinin and MTT assays. These results should aid the development of new drugs for the prevention and control of influenza virus infections.Entities:
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Year: 2007 PMID： 18078808 DOI： 10.1016/j.bbrc.2007.11.183
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575