BACKGROUND: Gene expression profiling of normal receptive endometrium has been characterized, but intrinsic defects in endometrial gene expression associated with implantation failure have not been reported. METHODS: Women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group) or had at least one successful cycle (Group B, control group) and spontaneously fertile women (Group C, normal fertility group) were recruited. All were treated with exogenous estradiol and progesterone to induce an endometrial cycle, and an endometrial biopsy was taken on the seventh day of progesterone administration. RNA from each sample was analysed by cDNA microarrays to identify differentially expressed genes between groups. RESULTS: 63 transcripts were differentially expressed (>or=2-fold) between Groups A and B, of which 16 were subjected to real time RT-PCR. Eleven of these were significantly decreased in Group A with regard to Groups B and C. Among the dysregulated genes were MMP-7, CXCR4, PAEP and C4BPA. CONCLUSIONS: Repeated implantation failure in some oocyte recipients is associated with an intrinsic defect in the expression of multiple genes in their endometrium. Significantly decreased levels of several transcripts in endometria without manifest abnormalities is demonstrated for the first time and shown to be associated with implantation failure.
BACKGROUND: Gene expression profiling of normal receptive endometrium has been characterized, but intrinsic defects in endometrial gene expression associated with implantation failure have not been reported. METHODS:Women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group) or had at least one successful cycle (Group B, control group) and spontaneously fertile women (Group C, normal fertility group) were recruited. All were treated with exogenous estradiol and progesterone to induce an endometrial cycle, and an endometrial biopsy was taken on the seventh day of progesterone administration. RNA from each sample was analysed by cDNA microarrays to identify differentially expressed genes between groups. RESULTS: 63 transcripts were differentially expressed (>or=2-fold) between Groups A and B, of which 16 were subjected to real time RT-PCR. Eleven of these were significantly decreased in Group A with regard to Groups B and C. Among the dysregulated genes were MMP-7, CXCR4, PAEP and C4BPA. CONCLUSIONS:Repeated implantation failure in some oocyte recipients is associated with an intrinsic defect in the expression of multiple genes in their endometrium. Significantly decreased levels of several transcripts in endometria without manifest abnormalities is demonstrated for the first time and shown to be associated with implantation failure.
Authors: Richard E Leach; Philip Jessmon; Christos Coutifaris; Michael Kruger; Evan R Myers; Rouba Ali-Fehmi; Sandra A Carson; Richard S Legro; William D Schlaff; Bruce R Carr; Michael P Steinkampf; Susan Silva; Phyllis C Leppert; Linda Giudice; Michael P Diamond; D Randall Armant Journal: Hum Reprod Date: 2012-01-02 Impact factor: 6.918
Authors: Paul B Miller; Brent A Parnell; Greta Bushnell; Nicholas Tallman; David A Forstein; H Lee Higdon; Jo Kitawaki; Bruce A Lessey Journal: Hum Reprod Date: 2012-01-13 Impact factor: 6.918
Authors: Ariane Germeyer; Michael von Wolff; Julia Jauckus; Thomas Strowitzki; Tanuj Sharma; Anna T Grazul-Bilska Journal: Reprod Biol Endocrinol Date: 2010-06-21 Impact factor: 5.211
Authors: Stefan Bauersachs; Heinrich Bollwein; Katharina S Weber; Karen Wagener; Miguel Blanco Journal: BMC Genomics Date: 2021-05-22 Impact factor: 3.969