OBJECTIVE: To study the incidence of asymptomatic primary dengue infections among children and reactivity against other flaviviruses. METHODS: A total of 216 children, who had no dengue-specific IgG antibodies during a serosurvey in 2003 were re-examined 23 months later to determine if seroconversion had occurred. Dengue-specific IgG was demonstrated with enzyme-linked immunosorbent assay (ELISA) and reactivity patterns against other flaviviruses were assessed by using immunofluorescence assay (IFA). RESULTS: Sixty-six children had seroconverted for dengue virus-specific IgG; the true annual incidence of primary dengue was thus 17.3% (95% CI: 13.8-21.4). Japanese Encephalitis virus (JEV)-specific IgG antibodies were detected by IFA among three (4.6%) samples that showed seroconversion in the dengue ELISA, because of cross-reactivity. CONCLUSION: Our findings highlight the high incidence of dengue among Vietnamese children; JEV infections are rare. The true annual incidence of dengue can be estimated with a single cross-sectional seroprevalence survey.
OBJECTIVE: To study the incidence of asymptomatic primary dengue infections among children and reactivity against other flaviviruses. METHODS: A total of 216 children, who had no dengue-specific IgG antibodies during a serosurvey in 2003 were re-examined 23 months later to determine if seroconversion had occurred. Dengue-specific IgG was demonstrated with enzyme-linked immunosorbent assay (ELISA) and reactivity patterns against other flaviviruses were assessed by using immunofluorescence assay (IFA). RESULTS: Sixty-six children had seroconverted for dengue virus-specific IgG; the true annual incidence of primary dengue was thus 17.3% (95% CI: 13.8-21.4). Japanese Encephalitis virus (JEV)-specific IgG antibodies were detected by IFA among three (4.6%) samples that showed seroconversion in the dengue ELISA, because of cross-reactivity. CONCLUSION: Our findings highlight the high incidence of dengue among Vietnamese children; JEVinfections are rare. The true annual incidence of dengue can be estimated with a single cross-sectional seroprevalence survey.
Authors: Khoa T D Thai; Hiroshi Nishiura; Phuong Lan Hoang; Nga Thanh Thi Tran; Giao Trong Phan; Hung Quoc Le; Binh Quang Tran; Nam Van Nguyen; Peter J de Vries Journal: PLoS Negl Trop Dis Date: 2011-06-21
Authors: Khoa T D Thai; Hoang Lan Phuong; Tran Thi Thanh Nga; Phan Trong Giao; Le Quoc Hung; Nguyen Van Nam; Tran Quang Binh; Cameron Simmons; Jeremy Farrar; Tran Thinh Hien; H Rogier van Doorn; Menno D de Jong; Peter J de Vries Journal: J Infect Date: 2010-01-18 Impact factor: 6.072
Authors: Juliana Pérez-Pérez; Víctor Hugo Peña-García; Arley Calle-Tobón; Marcela Quimbayo-Forero; Raúl Rojo; Enrique Henao; Talya Shragai; Guillermo Rúa-Uribe Journal: Int J Environ Res Public Health Date: 2021-06-06 Impact factor: 3.390