OBJECTIVE: To prepare puerarin liposome and study its oral absorption in rat. METHODS: Liposome was prepared through the way of film dispersion-ultrasonic. Free puerarin in liposome suspension was separated from liposome through ultrafiltration, and then encapsulation ratio of liposome was determined. Micro-morphology of liposme particles was observed under electronic transmission microscope. Puerarin concentration in blood was determined by HPLC. RESULTS: The encapsulation ratio of puerarin in liposome was 53%, and liposome particles were global or elliptical. The diameter range of liposome particies was from 50 nm to 300 nm. The relative availability of puerarin liposme suspension to puerarin solution was 168%. CONCLUSION: Liposome as a drug carrier can enhance the oral absorption of puerarin in rat.
OBJECTIVE: To prepare puerarin liposome and study its oral absorption in rat. METHODS: Liposome was prepared through the way of film dispersion-ultrasonic. Free puerarin in liposome suspension was separated from liposome through ultrafiltration, and then encapsulation ratio of liposome was determined. Micro-morphology of liposme particles was observed under electronic transmission microscope. Puerarin concentration in blood was determined by HPLC. RESULTS: The encapsulation ratio of puerarin in liposome was 53%, and liposome particles were global or elliptical. The diameter range of liposome particies was from 50 nm to 300 nm. The relative availability of puerarin liposme suspension to puerarin solution was 168%. CONCLUSION: Liposome as a drug carrier can enhance the oral absorption of puerarin in rat.