Juan Guo1, Chang-xun Chen. 1. Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. guojuan338@hotmail.com
Abstract
OBJECTIVE: To approach the influnce of Tinglizi on some neuroendocrine factors and type I and III collagen in ventricular remodeling induced by Abdominal Aortic Banding (AAB) in rats. METHODS: Myocardial hypertrophy ventricular remodeling model was induced by abdominal aortic banding (AAB) in rats. After 30 day treatment, the systolic blood pressure (SBP) diastolic blood pressure (DBP) were measured; Then the ratios of LVW/BW (left ventricle weight/body weight), HW/BW (heart weight/body weight) were calculated; The Angiotensin II (Ang II) and Endothelin (ET-1) content in heart tissue and the Aldosterone (ALD) concentration of blood plasma were determined by radioimmunoassay. The content of type I and III collagen in myocardium were determined using immunohistochemical analysis. Hydroxy proline content in heart tissue was measured by hydrolysis method. RESULTS: The experimental data demonstrated that Tinglizi could decrease SBP, DBP and the cardiac indexes of LVW/BW and HW/BW, significantly reduce the content of ANg II, ALD and ET, decrease the total collagen content and type I and III collagen (P<0.05). CONCLUSION: Tiglizi can significantly attenuate the experimental ventricular remodeling; the mechanism is related with its ability to inhibit the activation of rennin-angiontensin-aldosterone system (RAAS) and systema nervosum sympatheticum (SNS), decrease the content of neuroendocrine factors (Ang II , ET, ALD).
OBJECTIVE: To approach the influnce of Tinglizi on some neuroendocrine factors and type I and III collagen in ventricular remodeling induced by Abdominal Aortic Banding (AAB) in rats. METHODS:Myocardial hypertrophy ventricular remodeling model was induced by abdominal aortic banding (AAB) in rats. After 30 day treatment, the systolic blood pressure (SBP) diastolic blood pressure (DBP) were measured; Then the ratios of LVW/BW (left ventricle weight/body weight), HW/BW (heart weight/body weight) were calculated; The Angiotensin II (Ang II) and Endothelin (ET-1) content in heart tissue and the Aldosterone (ALD) concentration of blood plasma were determined by radioimmunoassay. The content of type I and III collagen in myocardium were determined using immunohistochemical analysis. Hydroxy proline content in heart tissue was measured by hydrolysis method. RESULTS: The experimental data demonstrated that Tinglizi could decrease SBP, DBP and the cardiac indexes of LVW/BW and HW/BW, significantly reduce the content of ANg II, ALD and ET, decrease the total collagen content and type I and III collagen (P<0.05). CONCLUSION: Tiglizi can significantly attenuate the experimental ventricular remodeling; the mechanism is related with its ability to inhibit the activation of rennin-angiontensin-aldosterone system (RAAS) and systema nervosum sympatheticum (SNS), decrease the content of neuroendocrine factors (Ang II , ET, ALD).