OBJECTIVE: To select the microorganism which can hydrolyze dioscin to diosgenin. METHODS: The microorganism were selected from the surface of rhizome, rhizosphere soil, the inside of the leaves and rhizome of Dioscorea zingiberensis C. H. Wright. Diosgenin was identified by thin-layer chromatography and HPLC. RESULTS: The microorganism which could hydrolyze dioscin from the experiment were identified as Aspergillus sp and Alternaria sp. Characteristics of enzymes production and fermentation technology of Aspergillus No. 1 were also studied primarily. CONCLUSION: The Aspergillus strain No. 1 can secret enzyme to hydrolyze dioscin into diosgenin effectively.
OBJECTIVE: To select the microorganism which can hydrolyze dioscin to diosgenin. METHODS: The microorganism were selected from the surface of rhizome, rhizosphere soil, the inside of the leaves and rhizome of Dioscorea zingiberensis C. H. Wright. Diosgenin was identified by thin-layer chromatography and HPLC. RESULTS: The microorganism which could hydrolyze dioscin from the experiment were identified as Aspergillus sp and Alternaria sp. Characteristics of enzymes production and fermentation technology of Aspergillus No. 1 were also studied primarily. CONCLUSION: The Aspergillus strain No. 1 can secret enzyme to hydrolyze dioscin into diosgenin effectively.