AIM: To investigate the safety of beta-L-D4A on DNA polymerase alpha. METHODS: Ion exchange chromatography was used to separate DNA polymerase alpha from crude extract of human Hela cells. Detailed kinetic parameters were determined for beta-L-D4A against DNA polymerase alpha. RESULTS: DNA polymerase alpha was purified with 4% yield and 31000 units/mg specific activity. The Michaelis constant (Km = 3.22 micromol/L), 50% inhibition concentration (IC50 = 178.49 micromol/L) and inhibition constant (Ki = 126 micromol/L) of beta-L-D4A were determined by kinetic analysis. CONCLUSION: beta-L-D4A is a more safe nucleoside for hepatitis B virus infection with a lower host toxicity.
AIM: To investigate the safety of beta-L-D4A on DNA polymerase alpha. METHODS: Ion exchange chromatography was used to separate DNA polymerase alpha from crude extract of human Hela cells. Detailed kinetic parameters were determined for beta-L-D4A against DNA polymerase alpha. RESULTS:DNA polymerase alpha was purified with 4% yield and 31000 units/mg specific activity. The Michaelis constant (Km = 3.22 micromol/L), 50% inhibition concentration (IC50 = 178.49 micromol/L) and inhibition constant (Ki = 126 micromol/L) of beta-L-D4A were determined by kinetic analysis. CONCLUSION:beta-L-D4A is a more safe nucleoside for hepatitis B virus infection with a lower host toxicity.
Authors: Jeff Beckman; Kristi Kincaid; Michal Hocek; Thomas Spratt; Joachim Engels; Richard Cosstick; Robert D Kuchta Journal: Biochemistry Date: 2007-01-16 Impact factor: 3.162