Literature DB >> 18066303

Time-resolved DNA-microarray reading by an intensified CCD for ultimate sensitivity.

G Valentini, C D'Andrea, D Comelli, A Pifferi, P Taroni, A Torricelli, R Cubeddu, C Battaglia, C Consolandi, G Salani, L Rossi-Bernardi, G De Bellis.   

Abstract

We describe a novel technique for DNA-microarray reading based on time-resolved fluorescence measurements. We used an intensified CCD camera with picosecond resolution to acquire a set of time-delayed fluorescence images from a mutation DNA microarray marked with cyanine 3. We measured the fluorescence lifetimes of the marker and the background separately, and we used this information to calculate the amplitude map of the marker, starting from the time-delayed images. This procedure allowed us to identify hybridized spots that are not visible in fluorescence images acquired with continuous-wave detection.

Entities:  

Year:  2000        PMID： 18066303     DOI： 10.1364/ol.25.001648

Source DB:  PubMed          Journal:  Opt Lett        ISSN： 0146-9592            Impact factor:   3.776


  4 in total

Review 1.  Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment.

Authors:  Paolo P Provenzano; Kevin W Eliceiri; Patricia J Keely
Journal:  Clin Exp Metastasis       Date:  2008-09-03       Impact factor: 5.150

2.  Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager.

Authors:  Gerard Giraud; Holger Schulze; Day-Uei Li; Till T Bachmann; Jason Crain; David Tyndall; Justin Richardson; Richard Walker; David Stoppa; Edoardo Charbon; Robert Henderson; Jochen Arlt
Journal:  Biomed Opt Express       Date:  2010-11-04       Impact factor: 3.732

3.  FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ.

Authors:  Sunil Kumar; Dominic Alibhai; Anca Margineanu; Romain Laine; Gordon Kennedy; James McGinty; Sean Warren; Douglas Kelly; Yuriy Alexandrov; Ian Munro; Clifford Talbot; Daniel W Stuckey; Christopher Kimberly; Bertrand Viellerobe; Francois Lacombe; Eric W-F Lam; Harriet Taylor; Margaret J Dallman; Gordon Stamp; Edward J Murray; Frank Stuhmeier; Alessandro Sardini; Matilda Katan; Daniel S Elson; Mark A A Neil; Chris Dunsby; Paul M W French
Journal:  Chemphyschem       Date:  2011-02-17       Impact factor: 3.102

4.  Fluorescence lifetime imaging of quantum dot labeled DNA microarrays.

Authors:  Gerard Giraud; Holger Schulze; Till T Bachmann; Colin J Campbell; Andrew R Mount; Peter Ghazal; Mizanur R Khondoker; Alan J Ross; Stuart W J Ember; Ilenia Ciani; Chaker Tlili; Anthony J Walton; Jonathan G Terry; Jason Crain
Journal:  Int J Mol Sci       Date:  2009-04-24       Impact factor: 6.208
  4 in total

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