BACKGROUND: Clinical trials of measles vaccination administered as aerosol are planned with the aim of obtaining licensure. Measles antibody levels will be measured using the plaque reduction neutralization test (PRNT) to assess antibody responses as a surrogate marker of efficacy. METHODS: A working group examined laboratory protocols for measles PRNT in use at three reference centres and agreed to a standardised procedure, which was subsequently validated. RESULTS: Assay validation showed quantitative results varied approximately threefold both within and between assays. The lower limit of detection was approximately 20milliInternational Units/mL. CONCLUSIONS: A standardised laboratory protocol for measles PRNT was established and validated for use in clinical trials of aerosolized measles vaccines.
BACKGROUND: Clinical trials of measles vaccination administered as aerosol are planned with the aim of obtaining licensure. Measles antibody levels will be measured using the plaque reduction neutralization test (PRNT) to assess antibody responses as a surrogate marker of efficacy. METHODS: A working group examined laboratory protocols for measles PRNT in use at three reference centres and agreed to a standardised procedure, which was subsequently validated. RESULTS: Assay validation showed quantitative results varied approximately threefold both within and between assays. The lower limit of detection was approximately 20milliInternational Units/mL. CONCLUSIONS: A standardised laboratory protocol for measles PRNT was established and validated for use in clinical trials of aerosolized measles vaccines.
Authors: Benjamin J Umlauf; Iana H Haralambieva; Inna G Ovsyannikova; Richard B Kennedy; V Shane Pankratz; Robert M Jacobson; Gregory A Poland Journal: Viral Immunol Date: 2012-01-12 Impact factor: 2.257
Authors: Wen-Hsuan Lin; Diane E Griffin; Paul A Rota; Mark Papania; Stephen P Cape; David Bennett; Brian Quinn; Robert E Sievers; Charles Shermer; Kenneth Powell; Robert J Adams; Steven Godin; Scott Winston Journal: Proc Natl Acad Sci U S A Date: 2011-01-31 Impact factor: 11.205
Authors: George K Siberry; Kunjal Patel; William J Bellini; Brad Karalius; Murli U Purswani; Sandra K Burchett; William A Meyer; Sun Bae Sowers; Angela Ellis; Russell B Van Dyke Journal: Clin Infect Dis Date: 2015-06-09 Impact factor: 9.079