| Literature DB >> 18060384 |
Guo Xia Zhang1, Gui Xiang Peng, En Tao Wang, Hui Yan, Qing Hua Yuan, Wu Zhang, Xu Lou, Hui Wu, Zhi Yuan Tan.
Abstract
Twenty-three nitrogen-fixing bacteria were isolated from surface-sterilized stems and roots of wild rice Oryza rufipogon. Four clusters were defined among these bacteria by SDS-PAGE protein patterns and further confirmed by IS-PCR finger-printing analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the representative strains LS 8 and LS 18 of cluster II formed a monophyletic group sharing 94.0-97.3% similarities with defined enterobacterial species within the genera Salmonella, Citrobacter, Pantoea, Klebsiella, and Enterobacter. DNA-DNA hybridization, physiological, biochemical tests, and cell morphology also revealed that these strains could be differentiated from the related enterobacterial species. Based upon these results, we propose Phytobacter diazotrophicus gen. nov., sp. nov. to the bacterial group represented by strains LS 8 and LS 18. The type strain is LS 8(T) (=DSM 17806(T) = LMG 23328(T) = CGMCC 1.5339(T)). The DNA G+C content of strain LS 8(T) is 58.6 +/- 0.5 mol%.Entities:
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Year: 2007 PMID： 18060384 DOI： 10.1007/s00203-007-0333-7
Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552