Literature DB >> 18057126

Evaluation of the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for detection of influenza A and influenza B viruses and respiratory syncytial viruses in children.

Jérôme Legoff1, Rachid Kara, Florence Moulin, Ali Si-Mohamed, Anne Krivine, Laurent Bélec, Pierre Lebon.   

Abstract

We evaluated the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for the detection of influenza A and influenza B viruses and respiratory syncytial viruses from 353 pediatric nasopharyngeal aspirates. As assessed by comparison with the results of immunofluorescence testing and cell culture, the specificity and the sensitivity of the ProFlu-1 assay ranged from 97% to 100%. In addition, the ProFlu-1 assay amplified 9% of samples not detected by conventional methods.

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Year:  2007        PMID： 18057126      PMCID： PMC2238076          DOI： 10.1128/JCM.00959-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Effect of rapid viral diagnosis on the management of children hospitalized with lower respiratory tract infection.

Authors:  P M Adcock; G G Stout; M A Hauck; G S Marshall
Journal:  Pediatr Infect Dis J       Date:  1997-09       Impact factor: 2.129

2.  Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients.

Authors:  P C Woo; S S Chiu; W H Seto; M Peiris
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

3.  High prevalence of respiratory viral infections in patients hospitalized in an intensive care unit for acute respiratory infections as detected by nucleic acid-based assays.

Authors:  Jérôme Legoff; Emmanuel Guérot; Angélique Ndjoyi-Mbiguino; Mathieu Matta; Ali Si-Mohamed; Laurent Gutmann; Jean-Yves Fagon; Laurent Bélec
Journal:  J Clin Microbiol       Date:  2005-01       Impact factor: 5.948

4.  Evaluation of a monoclonal antibody pool for rapid diagnosis of respiratory viral infections.

Authors:  C Stout; M D Murphy; S Lawrence; S Julian
Journal:  J Clin Microbiol       Date:  1989-03       Impact factor: 5.948

5.  Performance characteristics of a rapid immunochromatographic assay for detection of influenza virus in children during the 2003 to 2004 influenza season.

Authors:  Andrea T Cruz; Andreea C Cazacu; Latresha J McBride; Jewel M Greer; Gail J Demmler
Journal:  Ann Emerg Med       Date:  2005-12-20       Impact factor: 5.721

6.  Rapid and quantitative detection of human adenovirus DNA by real-time PCR.

Authors:  Albert Heim; Carmen Ebnet; Gabi Harste; Patricia Pring-Akerblom
Journal:  J Med Virol       Date:  2003-06       Impact factor: 2.327

Review 7.  Lower respiratory infections in children.

Authors:  Jean E Klig; Lei Chen
Journal:  Curr Opin Pediatr       Date:  2003-02       Impact factor: 2.856

8.  Replication of respiratory viruses, particularly influenza virus, rhinovirus, and coronavirus in HuH7 hepatocarcinoma cell line.

Authors:  François Freymuth; Astrid Vabret; Flore Rozenberg; Julia Dina; Joëlle Petitjean; Stéphanie Gouarin; Loïc Legrand; Sandrine Corbet; Jacques Brouard; Pierre Lebon
Journal:  J Med Virol       Date:  2005-10       Impact factor: 2.327

9.  Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses.

Authors:  S Bellau-Pujol; A Vabret; L Legrand; J Dina; S Gouarin; J Petitjean-Lecherbonnier; B Pozzetto; C Ginevra; F Freymuth
Journal:  J Virol Methods       Date:  2005-06       Impact factor: 2.014

10.  Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children.

Authors:  Jane Kuypers; Nancy Wright; Rhoda Morrow
Journal:  J Clin Virol       Date:  2004-10       Impact factor: 3.168
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  22 in total

1.  Viral detection using DNA functionalized gold filaments.

Authors:  Jonas W Perez; Frederick R Haselton; David W Wright
Journal:  Analyst       Date:  2009-05-15       Impact factor: 4.616

2.  Evaluation of Alere i Influenza A&B for rapid detection of influenza viruses A and B.

Authors:  Shuping Nie; Richard B Roth; Jeffrey Stiles; Albina Mikhlina; Xuedong Lu; Yi-Wei Tang; N Esther Babady
Journal:  J Clin Microbiol       Date:  2014-07-02       Impact factor: 5.948

3.  Evaluation of three influenza A and B real-time reverse transcription-PCR assays and a new 2009 H1N1 assay for detection of influenza viruses.

Authors:  Suresh B Selvaraju; Rangaraj Selvarangan
Journal:  J Clin Microbiol       Date:  2010-09-15       Impact factor: 5.948

4.  Real-time detection of influenza a, influenza B, and respiratory syncytial virus a and B in respiratory specimens by use of nanoparticle probes.

Authors:  Paul J Jannetto; Blake W Buchan; Kimberly A Vaughan; Joellen S Ledford; Dennis K Anderson; Donald C Henley; Neil B Quigley; Nathan A Ledeboer
Journal:  J Clin Microbiol       Date:  2010-09-08       Impact factor: 5.948

Review 5.  Detection of respiratory viruses by molecular methods.

Authors:  James B Mahony
Journal:  Clin Microbiol Rev       Date:  2008-10       Impact factor: 26.132

6.  Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness.

Authors:  Elektra Szewczuk; Kiran Thapa; Terry Anninos; Kenneth McPhie; Geoff Higgins; Dominic E Dwyer; Keith K Stanley; Jonathan R Iredell
Journal:  BMC Infect Dis       Date:  2010-05-11       Impact factor: 3.090

7.  Clinical accuracy of a PLEX-ID flu device for simultaneous detection and identification of influenza viruses A and B.

Authors:  Yi-Wei Tang; Kristin S Lowery; Alexandra Valsamakis; Virginia C Schaefer; James D Chappell; Jill White-Abell; Criziel D Quinn; Haijing Li; Cicely A Washington; Jenna Cromwell; Chantel M Giamanco; Michael Forman; Jeffery Holden; Richard E Rothman; Michelle L Parker; Elaine V Ortenberg; Lei Zhang; Yea-Lin Lin; Charlotte A Gaydos
Journal:  J Clin Microbiol       Date:  2012-10-17       Impact factor: 5.948

8.  Rapid semiautomated subtyping of influenza virus species during the 2009 swine origin influenza A H1N1 virus epidemic in Milwaukee, Wisconsin.

Authors:  Michael E Bose; Eric T Beck; Nate Ledeboer; Sue C Kehl; Lisa A Jurgens; Teresa Patitucci; Lorraine Witt; Elizabeth LaGue; Patrick Darga; Jie He; Jiang Fan; Swati Kumar; Kelly J Henrickson
Journal:  J Clin Microbiol       Date:  2009-07-29       Impact factor: 5.948

9.  Rapid multiplex reverse transcription-PCR typing of influenza A and B virus, and subtyping of influenza A virus into H1, 2, 3, 5, 7, 9, N1 (human), N1 (animal), N2, and N7, including typing of novel swine origin influenza A (H1N1) virus, during the 2009 outbreak in Milwaukee, Wisconsin.

Authors:  Jie He; Michael E Bose; Eric T Beck; Jiang Fan; Sagarika Tiwari; Jacob Metallo; Lisa A Jurgens; Sue C Kehl; Nathan Ledeboer; Swati Kumar; William Weisburg; Kelly J Henrickson
Journal:  J Clin Microbiol       Date:  2009-07-29       Impact factor: 5.948

10.  Comparison of viral isolation and multiplex real-time reverse transcription-PCR for confirmation of respiratory syncytial virus and influenza virus detection by antigen immunoassays.

Authors:  R S Liao; L L Tomalty; A Majury; D E Zoutman
Journal:  J Clin Microbiol       Date:  2009-01-07       Impact factor: 5.948
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