| Literature DB >> 18055276 |
Pedro L Silva1, Caroline P Passaro, Viviane R Cagido, Marcelo Bozza, Marisa Dolhnikoff, Elnara M Negri, Maina M B Morales, Vera L Capelozzi, Walter A Zin, Patricia R M Rocco.
Abstract
We developed a model of severe allergic inflammation and investigated the impact of airway and lung parenchyma remodelling on in vivo and in vitro respiratory mechanics. BALB/c mice were sensitized and challenged with ovalbumin in severe allergic inflammation (SA) group. The control group (C) received saline using the same protocol. Light and electron microscopy showed eosinophil and neutrophil infiltration and fibrosis in airway and lung parenchyma, mucus gland hyperplasia, and airway smooth muscle hypertrophy and hyperplasia in SA group. These morphological changes led to in vivo (resistive and viscoelastic pressures, and static elastance) and in vitro (tissue elastance and resistance) lung mechanical alterations. Airway responsiveness to methacholine was markedly enhanced in SA as compared with C group. Additionally, IL-4, IL-5, and IL-13 levels in the bronchoalveolar lavage fluid were higher in SA group. In conclusion, this model of severe allergic lung inflammation enabled us to directly assess the role of airway and lung parenchyma inflammation and remodelling on respiratory mechanics.Entities:
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Year: 2007 PMID： 18055276 DOI： 10.1016/j.resp.2007.10.009
Source DB: PubMed Journal: Respir Physiol Neurobiol ISSN： 1569-9048 Impact factor: 1.931