OBJECTIVE: To investigate the anti-inflammatory effect of n-3 eicosapentaenoic acid (EPA) compared with n-6 linoleic acid (LA) in an endometriosis rat model. We focused on the relationship between lipid metabolism and inflammatory reactions in endometriosis based on the hypothesis that a lipid intake imbalance is one of the factors responsible for the recent increase of endometriosis. DESIGN: Prospective, randomized experimental study. SETTING: Animal surgery laboratory in a university hospital. ANIMAL(S): Sprague-Dawley rats (female, 6 weeks old). INTERVENTION(S): Rats were fed a diet with EPA (n = 9) or with LA (n = 9) for 2 weeks. Two weeks after feeding, the uterus was autotransplanted to the peritoneum to construct an endometriosis model. Feeding was continued for a total of 6 weeks. Two and 4 weeks after autotransplantation, three rats of each group were killed and evaluated. MAIN OUTCOME MEASURE(S): Endometriotic lesions were morphologically evaluated and their fatty acid composition was examined. Gene expression in these tissues was evaluated by cDNA microarray analysis and quantative real-time reverse transcriptase-polymerase chain reaction (RT-PCR). RESULT(S): In the EPA group, the n-3:n-6 ratio in each tissue significantly increased and the thickening of the interstitium, an active site for inflammation in endometriosis, was significantly suppressed (0.30 +/- 0.09 mm [EPA group] vs. 0.77 +/- 0.23 mm [LA group]). The mRNA of metalloproteinases, interleukin-1beta, interleukin-1r, prostaglandin E synthase (Ptges), and nuclear factor (NF)-kappaB were reduced in the EPA group. CONCLUSION(S): EPA supplementation might be a valid strategy for the treatment of endometriosis.
OBJECTIVE: To investigate the anti-inflammatory effect of n-3 eicosapentaenoic acid (EPA) compared with n-6 linoleic acid (LA) in an endometriosisrat model. We focused on the relationship between lipid metabolism and inflammatory reactions in endometriosis based on the hypothesis that a lipid intake imbalance is one of the factors responsible for the recent increase of endometriosis. DESIGN: Prospective, randomized experimental study. SETTING: Animal surgery laboratory in a university hospital. ANIMAL(S): Sprague-Dawley rats (female, 6 weeks old). INTERVENTION(S): Rats were fed a diet with EPA (n = 9) or with LA (n = 9) for 2 weeks. Two weeks after feeding, the uterus was autotransplanted to the peritoneum to construct an endometriosis model. Feeding was continued for a total of 6 weeks. Two and 4 weeks after autotransplantation, three rats of each group were killed and evaluated. MAIN OUTCOME MEASURE(S): Endometriotic lesions were morphologically evaluated and their fatty acid composition was examined. Gene expression in these tissues was evaluated by cDNA microarray analysis and quantative real-time reverse transcriptase-polymerase chain reaction (RT-PCR). RESULT(S): In the EPA group, the n-3:n-6 ratio in each tissue significantly increased and the thickening of the interstitium, an active site for inflammation in endometriosis, was significantly suppressed (0.30 +/- 0.09 mm [EPA group] vs. 0.77 +/- 0.23 mm [LA group]). The mRNA of metalloproteinases, interleukin-1beta, interleukin-1r, prostaglandin E synthase (Ptges), and nuclear factor (NF)-kappaB were reduced in the EPA group. CONCLUSION(S): EPA supplementation might be a valid strategy for the treatment of endometriosis.
Authors: Korosh Khanaki; Mohammad Nouri; Ali M Ardekani; Alieh Ghassemzadeh; Vahideh Shahnazi; Mohammad R Sadeghi; Masoud Darabi; Amir Mehdizadeh; Homayon Dolatkhah; Abotaleb Saremi; Ali R Imani; Ali Rahimipour Journal: Iran Biomed J Date: 2012
Authors: Korosh Khanaki; Mohammad Reza Sadeghi; Mohammad Mehdi Akhondi; Masoud Darabi; Amir Mehdizadeh; Mahdi Shabani; Ali Rahimipour; Mohammad Nouri Journal: Iran J Reprod Med Date: 2014-11
Authors: Laura Buggio; Giussy Barbara; Federica Facchin; Maria Pina Frattaruolo; Giorgio Aimi; Nicola Berlanda Journal: Int J Womens Health Date: 2017-05-02
Authors: Korosh Khanaki; Ali Motavalizadeh Ardekani; Alieh Ghassemzadeh; Vahideh Shahnazi; Mohammad Reza Sadeghi; Masoud Darabi; Amir Mehdizadeh; Abotaleb Saremi; Jafar Soleimani-Rad; Ali Reza Imani; Mohammad Nouri; Ali Rahimipour Journal: Iran J Reprod Med Date: 2012-07
Authors: Ibtisam M Abokhrais; Philippa T K Saunders; Fiona C Denison; Ann Doust; Linda Williams; Andrew W Horne Journal: Pilot Feasibility Stud Date: 2018-04-25
Authors: Ibtisam M Abokhrais; Fiona C Denison; Lucy H R Whitaker; Philippa T K Saunders; Ann Doust; Linda J Williams; Andrew W Horne Journal: PLoS One Date: 2020-01-17 Impact factor: 3.240