AIM: To study whether macrophage migration inhibitory factor (MIF)-173 gene polymorphism correlates with inflammatory bowel disease (IBD) in Chinese Han population. METHODS: MIF-173 single nucleotide polymorphism (SNP) was genotyped by tetra-primer amplification refractory mutation system (ARMS) and restriction fragment length polymorphisms (RFLP)-PCR in 142 healthy subjects and 98 patients with inflammatory bowel disease (IBD). RESULTS: There were no discrepancies between the results obtained by tetra-primer ARMS and RFLP-PCR. The frequency of MIF-173 CC genotype was significantly higher in patients with ulcerative colitis (UC) 15.5% than in healthy individuals 5.6% (chi(2)=6.066, P=0.018, OR=3.067 and 95% CI=1.257-7.482). There was a trend towards a higher frequency of CC genotype among CD patients compared with healthy controls, however this did not attain the statistical significance (P=0.245). CONCLUSION: MIF-173 CC genotype may be associated with susceptibility to UC.
AIM: To study whether macrophage migration inhibitory factor (MIF)-173 gene polymorphism correlates with inflammatory bowel disease (IBD) in Chinese Han population. METHODS:MIF-173 single nucleotide polymorphism (SNP) was genotyped by tetra-primer amplification refractory mutation system (ARMS) and restriction fragment length polymorphisms (RFLP)-PCR in 142 healthy subjects and 98 patients with inflammatory bowel disease (IBD). RESULTS: There were no discrepancies between the results obtained by tetra-primer ARMS and RFLP-PCR. The frequency of MIF-173 CC genotype was significantly higher in patients with ulcerative colitis (UC) 15.5% than in healthy individuals 5.6% (chi(2)=6.066, P=0.018, OR=3.067 and 95% CI=1.257-7.482). There was a trend towards a higher frequency of CC genotype among CD patients compared with healthy controls, however this did not attain the statistical significance (P=0.245). CONCLUSION:MIF-173 CC genotype may be associated with susceptibility to UC.
Authors: James D Falvey; Robert W Bentley; Tony R Merriman; Mark B Hampton; Murray L Barclay; Richard B Gearry; Rebecca L Roberts Journal: World J Gastroenterol Date: 2013-10-21 Impact factor: 5.742
Authors: Lutz E Lehmann; Malte Book; Wolfgang Hartmann; Stefan U Weber; Jens-Christian Schewe; Sven Klaschik; Andreas Hoeft; Frank Stüber Journal: J Transl Med Date: 2009-11-26 Impact factor: 5.531