Literature DB >> 180531

Structure of a nicked DNA-protein complex isolated from simian virus 40: covalent attachment of the protein to DNA and nick specificity.

H Kasamatsu, M Wu.   

Abstract

A portion of the nicked circular DNA isolated from purified simian virus 40 contains a protein-DNA complex in which protein(s) is covalently attached to the end of a DNA single strand. (Nicked DNA is double-stranded DNA that contains at least one single-strand scission.) The protein was visualized by electron microscopy and labeled in vitro with 125I. The bond between the protein and the DNA is stable in alkali, 4 M guanidine-hydrochloride, 3.86 M hydroxylamine (pH 4,23), and in 98% formamide. Most of the molecules in the nicked circular DNA fraction contained one nick. The nick occurs on either of the two complementary strands; the specific nick sites on the two strands are staggered, but lie within a few hundred nucleotides of each other.

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Year:  1976        PMID： 180531      PMCID： PMC430424          DOI： 10.1073/pnas.73.6.1945

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  Relaxation complexes of poasmid DNA and protein. III. Association of protein with the 5' terminus of the broken DNA strand in the relaxed complex of plasmid ColE1.

Authors:  D G Guiney; D R Helinski
Journal:  J Biol Chem       Date:  1975-11-25       Impact factor: 5.157

2.  A circular DNA-protein complex adenoviruses and its possible role in DNA replication.

Authors:  A J Robinson; J D Bellett
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1975

3.  A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules.

Authors:  H J Vollenweider; J M Sogo; T Koller
Journal:  Proc Natl Acad Sci U S A       Date:  1975-01       Impact factor: 11.205

4.  Protein-SV40 DNA complex stable in high salt and sodium dodecyl sulfate.

Authors:  H Kasamatsu; M Wu
Journal:  Biochem Biophys Res Commun       Date:  1976-02-09       Impact factor: 3.575

5.  Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.

Authors:  P A Sharp; M T Hsu; E Otsubo; N Davidson
Journal:  J Mol Biol       Date:  1972-11-14       Impact factor: 5.469

6.  Strand-specific nick in open circular R-factor deoxyribonucleic acid: attachment of the linear strand to a proteinaceous cellular component.

Authors:  C F Morris; C L Hershberger; R Rownd
Journal:  J Bacteriol       Date:  1973-04       Impact factor: 3.490

7.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

8.  A circula DNA-protein complex from adenoviruses.

Authors:  A J Robinson; H B Younghusband; A J Bellett
Journal:  Virology       Date:  1973-11       Impact factor: 3.616

9.  Structure and composition of the adenovirus type 2 core.

Authors:  D T Brown; M Westphal; B T Burlingham; U Winterhoff; W Doerfler
Journal:  J Virol       Date:  1975-08       Impact factor: 5.103

10.  The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.

Authors:  A E Bolton; W M Hunter
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857
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  17 in total

1.  Genome-linked protein associated with the 5' termini of bacteriophage phi29 DNA.

Authors:  C O Yehle
Journal:  J Virol       Date:  1978-09       Impact factor: 5.103

2.  Conserved primary sequences of the DNA terminal proteins of five different human adenovirus groups.

Authors:  M Green; K Brackmann; W S Wold; M Cartas; H Thornton; J H Elder
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Identification of specific fragments containing the 5' end of poliovirus RNA after ribonuclease III digestion.

Authors:  T J Harris; J J Dunn; E Wimmer
Journal:  Nucleic Acids Res       Date:  1978-11       Impact factor: 16.971

4.  Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.

Authors:  J J Champoux
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

5.  Characterization of DNA-protein complexes from simian adenovirus SA7.

Authors:  M K Estes
Journal:  J Virol       Date:  1978-03       Impact factor: 5.103

6.  An electron microscopic method for the mapping of proteins attached to nucleic acids.

Authors:  M Wu; N Davidson
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

7.  Escherichia coli DNA topoisomerase I catalyzed linking of single-stranded rings of complementary base sequences.

Authors:  K Kirkegaard; J C Wang
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

8.  O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus.

Authors:  P G Rothberg; T J Harris; A Nomoto; E Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

9.  Origin of DNA replication in papovavirus chromatin is recognized by endogenous endonuclease.

Authors:  W Waldeck; B Föhring; K Chowdhury; P Gruss; G Sauer
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

10.  Interaction of polyoma and mouse DNAs. IV. Time course and extent of integration of polyoma DNA into mouse DNA during lytic infection.

Authors:  H Türler
Journal:  J Virol       Date:  1977-08       Impact factor: 5.103
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