BACKGROUND: Erythropoietin (Epo) was originally defined as a hematopoietic growth factor, but also has potent tissue-protective properties. The cytokine-modulating actions of Epo have received scant attention. We hypothesized that Epo significantly influences the in vitro cytokine production in both neonates and adults. METHODS: The effects of Epo were investigated using a standardized in vitro whole blood assay. Production of various cytokines was assessed by means of intracellular cytokine detection (IL-2, -6, -8, IFN-gamma and TNF-alpha) in preterm infants, term neonates and adults. Furthermore, synthesis of IL-4, -5 and -10 in adults was investigated via cytometric bead array. RESULTS: Epo significantly inhibits the production of various cytokines in preterm infants, term neonates and adults. In CD3+ lymphocytes, Epo predominantly decreases the number of IL-2-positive cells in all age groups. Similarly, in CD14+ cells, Epo significantly diminishes the number of IL-6- and TNF-alpha-producing cells. Furthermore, Epo significantly inhibits the synthesis of IL-4, IL-5 and IL-10 in adults. CONCLUSION: rhEpo has significant inhibitory potential on the production of various cytokines by leukocytes in preterm and term infants as well as in adults. The described effect likely contributes to the tissue protective properties of Epo.
BACKGROUND:Erythropoietin (Epo) was originally defined as a hematopoietic growth factor, but also has potent tissue-protective properties. The cytokine-modulating actions of Epo have received scant attention. We hypothesized that Epo significantly influences the in vitro cytokine production in both neonates and adults. METHODS: The effects of Epo were investigated using a standardized in vitro whole blood assay. Production of various cytokines was assessed by means of intracellular cytokine detection (IL-2, -6, -8, IFN-gamma and TNF-alpha) in preterm infants, term neonates and adults. Furthermore, synthesis of IL-4, -5 and -10 in adults was investigated via cytometric bead array. RESULTS:Epo significantly inhibits the production of various cytokines in preterm infants, term neonates and adults. In CD3+ lymphocytes, Epo predominantly decreases the number of IL-2-positive cells in all age groups. Similarly, in CD14+ cells, Epo significantly diminishes the number of IL-6- and TNF-alpha-producing cells. Furthermore, Epo significantly inhibits the synthesis of IL-4, IL-5 and IL-10 in adults. CONCLUSION: rhEpo has significant inhibitory potential on the production of various cytokines by leukocytes in preterm and term infants as well as in adults. The described effect likely contributes to the tissue protective properties of Epo.
Authors: Nancy Fathali; Robert P Ostrowski; Yu Hasegawa; Tim Lekic; Jiping Tang; John H Zhang Journal: Transl Stroke Res Date: 2013-04 Impact factor: 6.829
Authors: Janet E Orrock; Karuna Panchapakesan; Gilbert Vezina; Taeun Chang; Kari Harris; Yunfei Wang; Susan Knoblach; An N Massaro Journal: Pediatr Res Date: 2015-12-30 Impact factor: 3.756
Authors: Christina Irene Günter; Augustinus Bader; Ulf Dornseifer; Silvia Egert; Sebastian Dunda; Gerrit Grieb; Thomas Wolter; Norbert Pallua; Tobias von Wild; Frank Siemers; Peter Mailänder; Oliver Thamm; Carsten Ernert; Michael Steen; Reiner Sievers; Bert Reichert; Afshin Rahmanian-Schwarz; Hans Schaller; Bernd Hartmann; Max Otte; Victoria Kehl; Christian Ohmann; Wolfgang Jelkmann; Hans-Günther Machens Journal: Trials Date: 2013-05-03 Impact factor: 2.279