Literature DB >> 1805218

Purification of in vitro excysted Sarcocystis sporozoites by passage through a modified DE 52 anion-exchange column.

K Ono1, K Horn, A O Heydorn.   

Abstract

A method of purifying Sarcocystis sporozoites following in vitro excystation is described. After passage of sporozoites through a modified DE 52 anion-exchange cellulose column, the mean recovery was 88% for S. capracanis, 71% for S. hircicanis, 77% for S. ovicanis and 74% for S. arieticanis. Most of the sporozoites were motile, and the parasite suspension was free of excystation debris.

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Year:  1991        PMID： 1805218     DOI： 10.1007/BF00928689

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  10 in total

1.  [Differentiation of Sarcocystis muris and S. dispersa infections of the mouse using isoelectric focusing and immune assays].

Authors:  I Reiter; A Mareis
Journal:  Dtsch Tierarztl Wochenschr       Date:  1986-10-08

2.  Improved method for high-yield excystation and purification of infective sporozoites of Eimeria spp.

Authors:  J E Hosek; K S Todd; M S Kuhlenschmidt
Journal:  J Protozool       Date:  1988-11

3.  Identification of antigens of Sarcocystis cruzi sporozoites, merozoites, and bradyzoites with monoclonal antibodies.

Authors:  D E Burgess; C A Speer; D W Reduker
Journal:  J Parasitol       Date:  1988-10       Impact factor: 1.276

4.  Differentiation of Sarcocystis species in European sheep by isoelectric focusing.

Authors:  A M Tenter; M R Johnson; G L Zimmerman
Journal:  Parasitol Res       Date:  1989       Impact factor: 2.289

5.  Purification of Eimeria sporozoites by DE-52 anion exchange chromatography.

Authors:  D M Schmatz; M S Crane; P K Murray
Journal:  J Protozool       Date:  1984-02

6.  Effects of nylon wool purification on infectivity and antigenicity of Eimeria falciformis sporozoites and merozoites.

Authors:  R A Larsen; J E Kyle; W M Whitmire; C A Speer
Journal:  J Parasitol       Date:  1984-08       Impact factor: 1.276

7.  [Chemoprophylaxis and therapy of acute sarcosporidiosis].

Authors:  A O Heydorn; S Haralambidis; F R Matuschka
Journal:  Berl Munch Tierarztl Wochenschr       Date:  1981-06-01       Impact factor: 0.328

8.  Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.

Authors:  S M Lanham; D G Godfrey
Journal:  Exp Parasitol       Date:  1970-12       Impact factor: 2.011

9.  Trypanosoma brucei: detection of low parasitaemias in mice by a miniature anion-exchanger/centrifugation technique.

Authors:  W H Lumsden; C D Kimber; M Strange
Journal:  Trans R Soc Trop Med Hyg       Date:  1977       Impact factor: 2.184

10.  Biochemical and immunological characterization of major surface antigens of Sarcocystis muris and S. suicanis cyst merozoites.

Authors:  I Sommer; H Mehlhorn; W Rüger
Journal:  Parasitol Res       Date:  1991       Impact factor: 2.289
  10 in total
  2 in total

1.  Cross-reactivity of anti-Eimeria tenella antibody fragments on merozoites and sporozoites of different chicken Eimeria species.

Authors:  Reda E Khalafalla; Arwid Daugschies; Viktor Dyachenko
Journal:  Parasitol Res       Date:  2010-11-24       Impact factor: 2.289

2.  A comparison of sporozoite and cyst merozoite surface proteins of Sarcocystis.

Authors:  I Sommer; K Horn; A O Heydorn; H Mehlhorn; W Rüger
Journal:  Parasitol Res       Date:  1992       Impact factor: 2.289
  2 in total

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