Literature DB >> 1805214

Application of the western blotting procedure for the immunodiagnosis of human toxocariasis.

J F Magnaval1, R Fabre, P Maurières, J P Charlet, B de Larrard.   

Abstract

To improve the immunodiagnosis of human toxocaral disease, a sensitive and specific assay using the Western blotting procedure (WB) with excretory-secretory antigens from Toxocara canis larvae (TES) was developed and compared with the standard enzyme-linked immunosorbent assay method (TES-ELISA) using the same antigens. We tested groups of sera from laboratory animals or patients presenting with toxocariasis or other helminthic diseases and a group of sera from people dwelling in an area endemic for toxocariasis who exhibited hypereosinophilia. Statistically, the WB assay correlated well with TES-ELISA, but the former was more specific for banding patterns corresponding to low-molecular-weight fractions, thus avoiding problems of cross-reactivity with sera infected with other helminthic diseases.

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Year:  1991        PMID： 1805214     DOI： 10.1007/BF00928685

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  26 in total

1.  In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans.

Authors:  D H Savigny
Journal:  J Parasitol       Date:  1975-08       Impact factor: 1.276

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Journal:  J Parasitol       Date:  1956-08       Impact factor: 1.276

3.  Chronic eosinophilia due to visceral larva migrans; report of three cases.

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Journal:  Pediatrics       Date:  1952-01       Impact factor: 7.124

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Authors:  J F Magnaval
Journal:  Presse Med       Date:  1987-02-07       Impact factor: 1.228

5.  Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis.

Authors:  V C Tsang; J M Peralta; A R Simons
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

6.  Evaluation of serodiagnostic tests for visceral larva migrans.

Authors:  L Glickman; P Schantz; R Dombroske; R Cypess
Journal:  Am J Trop Med Hyg       Date:  1978-05       Impact factor: 2.345

7.  Analysis of Toxocara canis larval excretory-secretory antigens: physicochemical characterization and antibody recognition.

Authors:  J E Badley; R B Grieve; D D Bowman; L T Glickman; J H Rockey
Journal:  J Parasitol       Date:  1987-06       Impact factor: 1.276

8.  [Screening for Chagas disease by immunoenzymology: comparison of ELISA with immunofluorescence and indirect hemagglutination in 976 blood donors].

Authors:  J F Magnaval; B Brochier; J P Charlet; L Gonzaga Dos Santos; G Larrouy
Journal:  Rev Fr Transfus Immunohematol       Date:  1985-06

9.  An international study on the serological differential diagnosis of human cystic and alveolar echinococcosis.

Authors:  B Gottstein; P M Schantz; T Todorov; A G Saimot; P Jacquier
Journal:  Bull World Health Organ       Date:  1986       Impact factor: 9.408

10.  Visceral larva migrans in French adults: a new disease syndrome?

Authors:  L T Glickman; J F Magnaval; L M Domanski; F S Shofer; S S Lauria; B Gottstein; B Brochier
Journal:  Am J Epidemiol       Date:  1987-06       Impact factor: 4.897
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  56 in total

1.  Five cases of ocular toxocariasis confirmed by serology.

Authors:  S P Park; I Park; H Y Park; S U Lee; S Huh; J F Magnaval
Journal:  Korean J Parasitol       Date:  2000-12       Impact factor: 1.341

2.  Immunoblot for the detection of Ascaris suum-specific antibodies in patients with visceral larva migrans (VLM) syndrome.

Authors:  Renate Schneider; Andreas Obwaller; Herbert Auer
Journal:  Parasitol Res       Date:  2014-11-01       Impact factor: 2.289

3.  Rheumatoid purpura associated with toxocariasis.

Authors:  Anne-Pauline Bellanger; Jamal Bamoulid; Laurence Millon; Jean-Marc Chalopin; Philippe Humbert
Journal:  Can Fam Physician       Date:  2011-12       Impact factor: 3.275

4.  Toxocariasis masquerading as liver and lung metastatic nodules in patents with gastrointestinal cancer: clinicopathologic study of five cases.

Authors:  Sanghui Park; Yun Soo Kim; Yu Jin Kim; Sun Young Kyung; Jeong-Woong Park; Sung Hwan Jeong; Sang Pyo Lee
Journal:  Dig Dis Sci       Date:  2011-08-11       Impact factor: 3.199

5.  Pleural effusion and toxocariasis.

Authors:  T Jeanfaivre; B Cimon; N Tolstuchow; L de Gentile; D Chabasse; E Tuchais
Journal:  Thorax       Date:  1996-01       Impact factor: 9.139

6.  Development and evaluation of a sensitive and specific assay for diagnosis of human toxocariasis by use of three recombinant antigens (TES-26, TES-30USM, and TES-120).

Authors:  Suharni Mohamad; Norhaida Che Azmi; Rahmah Noordin
Journal:  J Clin Microbiol       Date:  2009-04-15       Impact factor: 5.948

7.  Seroprevalence of human toxocariasis in Denmark.

Authors:  Christen R Stensvold; Jakob Skov; Lone N Møller; Per M Jensen; Christian M O Kapel; Eskild Petersen; Henrik V Nielsen
Journal:  Clin Vaccine Immunol       Date:  2009-07-29

8.  A serological follow-up of toxocariasis patients after chemotherapy based on the detection of IgG, IgA, and IgE antibodies by enzyme-linked immunosorbent assay.

Authors:  Guita Rubinsky Elefant; Sumie Hoshino Shimizu; Maria Carmen Arroyo Sanchez; Cristina Miuki Abe Jacob; Antonio Walter Ferreira
Journal:  J Clin Lab Anal       Date:  2006       Impact factor: 2.352

9.  Epidemiologic approach to human toxocariasis in western France.

Authors:  B Gueglio; L de Gentile; J M Nguyen; J Achard; D Chabasse; M Marjolet
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

10.  Seroprevalence of toxocariasis among healthy people with eosinophilia.

Authors:  Yong Hun Kim; Sun Huh; Young Bae Chung
Journal:  Korean J Parasitol       Date:  2008-03       Impact factor: 1.341
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