[Development of amplification technology-based methods for tick-borne encephalitis virus detection and genotyping].

The development of methods for tick-borne encephalitis (TBE) virus genotyping, such as determination of nucleotide sequences, analysis of restriction fragment length polymorphism, polymerase chain reaction using genotype-specific probes with hybridization-fluorescence detection, is under consideration. The capacities and application area of varions typing techniques, such as TBE detection and genotyping in the field, autoptic, and cultural materials; study of circulating genotypes in different natural foci and in various viral vectors and carriers, are described. Each technique was tested using a large number of strains isolated from ill humans and ticks in different regions of the country and employed to investigate abundant materials.