| Literature DB >> 18048020 |
Karen E Duffy1, Roberta J Lamb, Lani R San Mateo, Jarrat L Jordan, Gabriela Canziani, Michael Brigham-Burke, Jeremy Korteweg, Mark Cunningham, Heena Solanki Beck, Jill Carton, Jill Giles-Komar, Cynthia Duchala, Robert T Sarisky, M Lamine Mbow.
Abstract
Toll-like receptors are a family of pattern-recognition receptors that contribute to the innate immune response. Toll-like receptor 3 (TLR3) signals in response to foreign, endogenous and synthetic ligands including viral dsRNA, bacterial RNA, mitochondrial RNA, endogenous necrotic cell mRNA and the synthetic dsRNA analog, poly(I:C). We have generated a monoclonal antibody (mAb CNTO2424) that recognizes the extracellular domain (ECD) of human TLR3 in a conformation-dependent manner. CNTO2424 down-regulates poly(I:C)-induced production of IL-6, IL-8, MCP-1, RANTES, and IP-10 in human lung epithelial cells. In addition, mAb CNTO2424 was able to interfere with the known TLR3-dependent signaling pathways, namely NF-kappaB, IRF-3/ISRE, and p38 MAPK. The generation of this neutralizing anti-TLR3 mAb provides a unique tool to better understand TLR3 signaling and potential cross-talk between TLR3 and other molecules.Entities:
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Year: 2007 PMID： 18048020 DOI： 10.1016/j.cellimm.2007.10.002
Source DB: PubMed Journal: Cell Immunol ISSN： 0008-8749 Impact factor: 4.868