| Literature DB >> 18046587 |
Belén Fernández1, Natividad Gálvez, Purificación Sánchez, Rafael Cuesta, Ruperto Bermejo, José M Domínguez-Vera.
Abstract
We simultaneously labeled ferritin with two Alexa Fluor fluorophores (AF350 and AF430). When both fluorophores label the same ferritin subunit, fluorescence resonance energy transfer (FRET) takes place from the excited AF350 to the acceptor AF430. By varying the number and the ratio of labeling fluorophores, we can modulate FRET such that the ferritin particles can exhibit multiple colors under UV illumination. Labeling of the ferritin shell does not affect the properties of the metallic core.Entities:
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Year: 2007 PMID: 18046587 DOI: 10.1007/s00775-007-0323-x
Source DB: PubMed Journal: J Biol Inorg Chem ISSN: 0949-8257 Impact factor: 3.358