BACKGROUND: Heart failure is characterized by limited exercise tolerance and by a skeletal muscle myopathy with atrophy and shift toward fast fibres. An inflammatory status with elevated pro-inflammatory cytokines and exaggerated free radicals production can worsen muscle damage. We have previously demonstrated in a model of heart failure, the monocrotaline treated rat, that oxidation of skeletal muscle actin, tropomyosin and myosin produces a reduction of contractile efficiency, which may further depress muscle function and exercise capacity. AIMS: To investigate the presence of oxidized myofibrillar proteins in skeletal muscle of CHF patients by means of the Oxyblot technique and to correlate it with exercise capacity. METHODS: We have analyzed skeletal muscle biopsies taken from six patients with class III-IV NYHA CHF and four control patients (peak VO(2) 12.8 +/- 1.9 vs. 29.7 +/- 1.7 ml/kg/min, p < 0.0001). RESULTS AND CONCLUSIONS: A correlation between degree of myofibrillar oxidation and exercise capacity measured as peak VO(2) was obtained. In the skeletal muscle of CHF patient there was a much higher level of myofibrillar protein oxidation as expressed by the Oxyblot/Red Ponceau (Oxy/RP) ratio as compared to controls (2.1 +/- 0.3 vs. 1.02 +/- 0.09, p < 0.0001). The VO(2)/Oxy/RP was significantly lower in the CHF patients. Higher levels of muscle oxidation were found in patients with lower exercise capacity with an inverse correlation between Oxyblot and VO(2) values (r (2) = 0.83).
BACKGROUND:Heart failure is characterized by limited exercise tolerance and by a skeletal muscle myopathy with atrophy and shift toward fast fibres. An inflammatory status with elevated pro-inflammatory cytokines and exaggerated free radicals production can worsen muscle damage. We have previously demonstrated in a model of heart failure, the monocrotaline treated rat, that oxidation of skeletal muscle actin, tropomyosin and myosin produces a reduction of contractile efficiency, which may further depress muscle function and exercise capacity. AIMS: To investigate the presence of oxidized myofibrillar proteins in skeletal muscle of CHFpatients by means of the Oxyblot technique and to correlate it with exercise capacity. METHODS: We have analyzed skeletal muscle biopsies taken from six patients with class III-IV NYHA CHF and four control patients (peak VO(2) 12.8 +/- 1.9 vs. 29.7 +/- 1.7 ml/kg/min, p < 0.0001). RESULTS AND CONCLUSIONS: A correlation between degree of myofibrillar oxidation and exercise capacity measured as peak VO(2) was obtained. In the skeletal muscle of CHFpatient there was a much higher level of myofibrillar protein oxidation as expressed by the Oxyblot/Red Ponceau (Oxy/RP) ratio as compared to controls (2.1 +/- 0.3 vs. 1.02 +/- 0.09, p < 0.0001). The VO(2)/Oxy/RP was significantly lower in the CHFpatients. Higher levels of muscle oxidation were found in patients with lower exercise capacity with an inverse correlation between Oxyblot and VO(2) values (r (2) = 0.83).
Authors: Rob C I Wüst; David S Myers; Rachel Stones; David Benoist; Philip A Robinson; John P Boyle; Chris Peers; Ed White; Harry B Rossiter Journal: Am J Physiol Heart Circ Physiol Date: 2011-10-28 Impact factor: 4.733
Authors: Francesco Agostini; Luciano Dalla Libera; Jörn Rittweger; Sara Mazzucco; Mihaela Jurdana; Igor B Mekjavic; Rado Pisot; Luisa Gorza; Marco Narici; Gianni Biolo Journal: J Physiol Date: 2010-10-20 Impact factor: 5.182
Authors: Raquel Santilone Bertaglia; Joyce Reissler; Francis Silva Lopes; Walter Luiz Garrido Cavalcante; Fernanda Regina Carani; Carlos Roberto Padovani; Sergio Augusto Rodrigues; Antônio Carlos Cigogna; Robson Francisco Carvalho; Ana Angélica Henrique Fernandes; Marcia Gallacci; Maeli Dal Pai Silva Journal: J Mol Histol Date: 2011-04-21 Impact factor: 2.611
Authors: Damien M Callahan; Mark S Miller; Andrew P Sweeny; Timothy W Tourville; James R Slauterbeck; Patrick D Savage; David W Maugan; Philip A Ades; Bruce D Beynnon; Michael J Toth Journal: J Physiol Date: 2014-07-18 Impact factor: 5.182
Authors: Karen Maes; Dries Testelmans; Pascal Cadot; Keith Deruisseau; Scott K Powers; Marc Decramer; Ghislaine Gayan-Ramirez Journal: Am J Respir Crit Care Med Date: 2008-10-10 Impact factor: 21.405