Polyethylenimine-DNA solid particles for gene delivery.

Polyethylenimine (PEI), a cationic polymer, was used to develop a non-viral vector for gene delivery. A simple, reproducible process is described with which to condense plasmid DNA with PEI. When prepared at the optimum charge ratio of 6.3 ( +/- ; PEI:DNA, 5:1 w/w), PEI-DNA complexes were 30-60 nm in diameter and excluded intercalating dyes from the plasmid DNA. The particles were stable for more than one month at 4 degrees C with respect to size and transfection activity. PEI-condensed DNA transfected a broad range of murine and human tumor cell lines (B16, Lewis Lung, SK-OV-3 and LS180) in vitro in the presence of fetal calf serum. Intraperitoneal administration of PEI-condensed DNA resulted in significant gene expression in a human ovarian cancer peritoneal xenograft model.