BACKGROUND AND PURPOSE: Low-dose radiotherapy (LD-RT) has a potent anti-inflammatory effect, and transforming growth factor (TGF)-beta(1) is a potential mediator of this effect. The objectives of this study were to characterize the in vivo effects of LD-RT on leukocyte recruitment over time, and its relationship with TGF-beta(1) production. MATERIALS AND METHODS: Mice were submitted to abdominal irradiation with a dose of 0.3 Gy, or to sham radiation and studied 5, 24, 48 or 72 h after irradiation. Four hours before the study a proinflammatory stimulus consisting of LPS or placebo was administered. Leukocyte-endothelial cell interactions in intestinal venules were assessed using intravital microscopy. Circulating levels and intestinal tissue production of TGF-beta(1) were determined. RESULTS: Compared to non-irradiated LPS-challenged group, the number of adherent leukocytes was significantly reduced 5, 24 and 48 h, but not 72 h after irradiation in LPS-challenged mice. Rolling leukocytes were significantly decreased at all time points analyzed. Plasma TGF-beta(1) levels were increased 5 and 24h after irradiation. Increased intestinal production during this period was corroborated by in vitro culture experiments. CONCLUSIONS: LD-RT has a sustained inhibitory effect on leukocyte recruitment for 48 h, which is initially associated with an increase in TGF-beta(1) intestinal production.
BACKGROUND AND PURPOSE: Low-dose radiotherapy (LD-RT) has a potent anti-inflammatory effect, and transforming growth factor (TGF)-beta(1) is a potential mediator of this effect. The objectives of this study were to characterize the in vivo effects of LD-RT on leukocyte recruitment over time, and its relationship with TGF-beta(1) production. MATERIALS AND METHODS:Mice were submitted to abdominal irradiation with a dose of 0.3 Gy, or to sham radiation and studied 5, 24, 48 or 72 h after irradiation. Four hours before the study a proinflammatory stimulus consisting of LPS or placebo was administered. Leukocyte-endothelial cell interactions in intestinal venules were assessed using intravital microscopy. Circulating levels and intestinal tissue production of TGF-beta(1) were determined. RESULTS: Compared to non-irradiated LPS-challenged group, the number of adherent leukocytes was significantly reduced 5, 24 and 48 h, but not 72 h after irradiation in LPS-challenged mice. Rolling leukocytes were significantly decreased at all time points analyzed. Plasma TGF-beta(1) levels were increased 5 and 24h after irradiation. Increased intestinal production during this period was corroborated by in vitro culture experiments. CONCLUSIONS:LD-RT has a sustained inhibitory effect on leukocyte recruitment for 48 h, which is initially associated with an increase in TGF-beta(1) intestinal production.
Authors: Elisabet Rodríguez-Tomàs; Johana C Acosta; Laura Torres-Royo; Gabriel De Febrer; Gerard Baiges-Gaya; Helena Castañé; Andrea Jiménez; Carlos Vasco; Pablo Araguas; Junior Gómez; Bárbara Malave; Miguel Árquez; David Calderón; Berta Piqué; Manel Algara; Ángel Montero; Josep M Simó; Xavier Gabaldó-Barrios; Sebastià Sabater; Jordi Camps; Jorge Joven; Meritxell Arenas Journal: Antioxidants (Basel) Date: 2022-06-16
Authors: M Algara; M Arenas; J Marin; I Vallverdu; P Fernandez-Letón; J Villar; G Fabrer; C Rubio; A Montero Journal: Clin Transl Radiat Oncol Date: 2020-06-15
Authors: M Arenas; M Algara; G De Febrer; C Rubio; X Sanz; M A de la Casa; C Vasco; J Marín; P Fernández-Letón; J Villar; L Torres-Royo; P Villares; I Membrive; J Acosta; M López-Cano; P Araguas; J Quera; F Rodríguez-Tomás; A Montero Journal: Strahlenther Onkol Date: 2021-07-06 Impact factor: 3.621