OBJECTIVES: To analyze the presence and distribution of collagen fibers and versican in human vocal fold lamina propria of fetal larynges. STUDY DESIGN: Cross sectional analysis of cadaveric vocal folds of human fetuses. METHODS: Seven fetal larynges obtained from 28- to 36-week-old fetuses were analyzed with the Picrosirius-polarization method, immunohistochemistry, and image analysis. RESULTS: Collagen fibers within the lamina propria exhibited a monolaminar distribution pattern and spatial arrangement in "wicker basket." Versican distribution was larger in the superficial and intermediate layers when compared to the deep layer. CONCLUSION: Our findings suggest that collagen and versican distribution and arrangement within the lamina propria in the developing fetus are important for vocalization at birth.
OBJECTIVES: To analyze the presence and distribution of collagen fibers and versican in human vocal fold lamina propria of fetal larynges. STUDY DESIGN: Cross sectional analysis of cadaveric vocal folds of human fetuses. METHODS: Seven fetal larynges obtained from 28- to 36-week-old fetuses were analyzed with the Picrosirius-polarization method, immunohistochemistry, and image analysis. RESULTS: Collagen fibers within the lamina propria exhibited a monolaminar distribution pattern and spatial arrangement in "wicker basket." Versican distribution was larger in the superficial and intermediate layers when compared to the deep layer. CONCLUSION: Our findings suggest that collagen and versican distribution and arrangement within the lamina propria in the developing fetus are important for vocalization at birth.
Authors: Alexandra J E Farran; Sean S Teller; Amit K Jha; Tong Jiao; Rohan A Hule; Rodney J Clifton; Darrin P Pochan; Randall L Duncan; Xinqiao Jia Journal: Tissue Eng Part A Date: 2010-04 Impact factor: 3.845