Yong-xia Tan1, Yu-hua Sun, Ruo-yun Chen. 1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Ministry of Education), Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
Abstract
OBJECTIVE: To study the chemical constituents in seeds of Cicer arietinum, so that to find bioactive natural products. METHOD: Dried and sprouted seeds of C. arietinum were extracted with ethanol of various concentrations respectively, then isolated and purified by silica gel, macroreticular resin D 101, Sephadex LH -20 gel column chromatography, and structures of compounds were identified by spectral analysis. RESULT: Nine compounds have been isolated and identified: 3-hydroxy-olean-12-ene (1), biochanin A-7-O-beta-D-glucoside (2), cerebroside (3), 1-ethyl-alpha-L-galactoside (4), uridine (5), adenosine (6), trytophan (7), biochanin A (8), fomononetin (9). CONCLUSION: Compounds 1, 3, 4, 6, 7 were isolated from genus Cicer for the first time.
OBJECTIVE: To study the chemical constituents in seeds of Cicer arietinum, so that to find bioactive natural products. METHOD: Dried and sprouted seeds of C. arietinum were extracted with ethanol of various concentrations respectively, then isolated and purified by silica gel, macroreticular resin D 101, Sephadex LH -20 gel column chromatography, and structures of compounds were identified by spectral analysis. RESULT: Nine compounds have been isolated and identified: 3-hydroxy-olean-12-ene (1), biochanin A-7-O-beta-D-glucoside (2), cerebroside (3), 1-ethyl-alpha-L-galactoside (4), uridine (5), adenosine (6), trytophan (7), biochanin A (8), fomononetin (9). CONCLUSION: Compounds 1, 3, 4, 6, 7 were isolated from genus Cicer for the first time.