| Literature DB >> 18025580 |
Tang Lianghua1, Xia Liming, Su Min, Guo Huaying.
Abstract
An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was about 28 kDa, determined by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzymatic resolution of racemic ibuprofen was carried out by the lipase from P. expansum PED-03, and the conversion reached 46% with excellent enantioselectivity(E > 200), which showed a good application potential in the production of optically pure ibuprofen.Entities:
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Year: 2007 PMID： 18025580 DOI： 10.1007/s12010-007-0043-2
Source DB: PubMed Journal: Appl Biochem Biotechnol ISSN： 0273-2289 Impact factor: 2.926