Literature DB >> 180247

The cleavage of polyoma virus DNA by restriction enzymes KpnI and PstI.

L V Crawford, A K Robbins.   

Abstract

The action of two restriction endonucleases on polyoma virus DNA has been examined and the sites at which they cleave the DNA located. One of the enzymes, KpnI from Klebsiella pneumoniae OK8, cleaves polyoma DNA twice at about 11-6 and 59-2% from the EcoRI site. The other enzyme, PstI from Providencia stuartii 164, cleaves polyoma DNA five times at about 14-8, 16-5, 32-6, 50-3 and 80-0% from the EcoRI site. Some of the cleavages produced by these enzymes alone, or in conjunction with other endonucleases, may be of use in the isolation of regions of particular interest from the virus DNA.

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Year:  1976        PMID： 180247     DOI： 10.1099/0022-1317-31-3-315

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  7 in total

1.  Characterization of the genome of the murine papovavirus K.

Authors:  M F Law; K K Takemoto; P M Howley
Journal:  J Virol       Date:  1979-04       Impact factor: 5.103

2.  Polyoma virus-specific RNA synthesis in an inducible line of polyoma virus-transformed rat cells.

Authors:  H Manor; R Kamen
Journal:  J Virol       Date:  1978-03       Impact factor: 5.103

3.  Physical map of the seven ribosomal RNA genes of Escherichia coli.

Authors:  I Boros; A Kiss; P Venetianer
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971

4.  XbaI, PstI, and BglII restriction enzyme maps of the two orientations of the varicella-zoster virus genome.

Authors:  A M Dumas; J L Geelen; M W Weststrate; P Wertheim; J van der Noordaa
Journal:  J Virol       Date:  1981-08       Impact factor: 5.103

5.  Free viral DNA in BK virus-induced hamster tumor cells.

Authors:  J ter Schegget; J Voves; A van Strien; J van der Noordaa
Journal:  J Virol       Date:  1980-08       Impact factor: 5.103

6.  Polyoma virus DNA: Sequence from the late region that specifies the leader sequence for late mRNA and codes for VP2, VP3, and the N-terminus of VP1.

Authors:  J R Arrand; E Soeda; J E Walsh; N Smolar; B E Griffin
Journal:  J Virol       Date:  1980-02       Impact factor: 5.103

7.  Evolutionary variants of simian virus 40 which are impaired in early lytic functions but transform nonpermissive cells.

Authors:  C J Sol; I Hassing; W Maris; C Walig; J van der Noordaa
Journal:  J Virol       Date:  1981-01       Impact factor: 5.103
  7 in total

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