OBJECTIVE: To investigate the association between angiotensin I-converting enzyme (ACE) gene and the levels of ACE and PAI-1 in Chinese Han patients with essential hypertension (EH) in Guangdong Province. METHODS: Polymerase chain reaction was used to examine the ACE genotype, colorimetry used to measure the serum ACE level, and spectrophotometric assay performed to examine the plasma PAI-1 level in 115 EH patients and 96 healthy controls in Guangdong Province. RESULTS: The ACE DD genotype and D allele frequencies were significantly higher in EH group than in the control group (P<0.05), and the EH patients also had significantly higher serum ACE level and plasma PAI-1 level than the control subjects (P<0.01). The serum ACE level was positively correlated with plasma PAI-1 level in both EH group and control group (r=0.7913 and 0.7806, respectively, P<0.01). In EH group, the patients with DD genotype showed significantly higher serum ACE and plasma PAI-1 levels than those with ID and II genotypes (P<0.01), and patients with ID genotype had significantly higher ACE and PAI-1 levels than those with II genotype (P<0.05). CONCLUSION: The DD genotype and D allele of ACE gene can be risk factors for essential hypertension in Chinese Han subjects in Guangdong Province, and the EH patients have elevated serum ACE and plasma PAI-1 levels. Increased ACE level due to DD polymorphism may play an important role in elevating plasma PAI-1 level. The genetic variation of ACE contributes to the balance of fibrinolytic pathway, which may be one of the pathological mechanisms linking the ACE I/D genotype and EH.
OBJECTIVE: To investigate the association between angiotensin I-converting enzyme (ACE) gene and the levels of ACE and PAI-1 in Chinese Han patients with essential hypertension (EH) in Guangdong Province. METHODS: Polymerase chain reaction was used to examine the ACE genotype, colorimetry used to measure the serum ACE level, and spectrophotometric assay performed to examine the plasma PAI-1 level in 115 EH patients and 96 healthy controls in Guangdong Province. RESULTS: The ACE DD genotype and D allele frequencies were significantly higher in EH group than in the control group (P<0.05), and the EH patients also had significantly higher serum ACE level and plasma PAI-1 level than the control subjects (P<0.01). The serum ACE level was positively correlated with plasma PAI-1 level in both EH group and control group (r=0.7913 and 0.7806, respectively, P<0.01). In EH group, the patients with DD genotype showed significantly higher serum ACE and plasma PAI-1 levels than those with ID and II genotypes (P<0.01), and patients with ID genotype had significantly higher ACE and PAI-1 levels than those with II genotype (P<0.05). CONCLUSION: The DD genotype and D allele of ACE gene can be risk factors for essential hypertension in Chinese Han subjects in Guangdong Province, and the EH patients have elevated serum ACE and plasma PAI-1 levels. Increased ACE level due to DD polymorphism may play an important role in elevating plasma PAI-1 level. The genetic variation of ACE contributes to the balance of fibrinolytic pathway, which may be one of the pathological mechanisms linking the ACE I/D genotype and EH.
Authors: Scott Miners; Emma Ashby; Shabnam Baig; Rachel Harrison; Hannah Tayler; Elizabeth Speedy; Jonathan A Prince; Seth Love; Patrick G Kehoe Journal: Am J Transl Res Date: 2009-01-18 Impact factor: 4.060
Authors: İbrahim Kaplan; Enver Sancaktar; Aydın Ece; Velat Şen; Nilgün Tekkeşin; Mustafa Kemal Basarali; Selvi Kelekci; Osman Evliyaoglu Journal: Med Sci Monit Date: 2014-09-28
Authors: Vana Kolovou; Olga Diakoumakou; Athanasia K Papazafiropoulou; Niki Katsiki; Elisabeth Fragopoulou; Ioannis Vasiliadis; Dimitris Degiannis; Leonidas Duntas; Smaragdi Antonopoulou; Genovefa Kolovou Journal: Open Cardiovasc Med J Date: 2018-07-31