| Literature DB >> 18022379 |
Earle Stellwagen1, Jason D Prantner, Nancy C Stellwagen.
Abstract
Capillary electrophoresis has been used to determine whether zwitterions contribute to the ionic strength of a solution, by measuring the mobility of a double-stranded DNA oligomer in cacodylate-buffered solutions containing various concentrations of the ionic salt tetraethylammonium chloride (TEA(+)Cl(-)) or the zwitterion tricine(+/-). The mobility of the DNA decreased as the square root of ionic strength, as expected from the Debye-Hückel-Onsager theory of electrophoresis, when TEA(+)Cl(-) was added to the buffer. However, the mobility was independent of the concentration of added tricine(+/-). Hence, zwitterions do not contribute to the ionic strength of a solution.Entities:
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Year: 2007 PMID: 18022379 PMCID: PMC2248451 DOI: 10.1016/j.ab.2007.10.038
Source DB: PubMed Journal: Anal Biochem ISSN: 0003-2697 Impact factor: 3.365