Distribution of mesenchymal stem cells in the area of tissue inflammation after transplantation of the cell material via different routes.

In experiments on male Wistar-Kyoto rats we studied the distribution of mesenchymal stem cells in intact body and in the presence of a focus of acute tissue inflammation. In healthy animals mesenchymal stem cells were transplanted intravenously. In the second case we used various routes of transplantation of mesenchymal stem cells: intravenous, into tissue adjacent to the inflammation focus, and into intact lobe of the damaged organ (prostate gland). Three weeks after transplantation, mesenchymal stem cells labeled with a fluorescent dye were detected in the bone marrow and intestine of intact animals. In case of inflammation focus, mesenchymal stem cells after transplantation migrated into the bone marrow, intestine, and prostate gland. After injection into the adjacent zone, these cells formed a compact agglomerate at the site of injection. After transplantation into the intact lobe of the prostate gland the cells migrated towards the inflammation focus. Thus, transplantation of mesenchymal stem cells into the venous blood is less traumatic and led to more uniform distribution of cells in the damaged tissue.