Literature DB >> 18007442

The preparation of fluorescence-quenched probes for use in the characterization of human factor Xa substrate binding domains.

Karen M Bromfield1, Julia Cianci, Peter J Duggan.   

Abstract

The preparation and characterization by LCMS of a library of 55 fluorescence- quenched peptides is described. The peptides bear a terminal anthranilamide fluorophore and a penultimate 2,4-dinitrophenyl-L-lysine quencher, and will be used to probe the substrate binding domain of the human blood coagulation enzyme, factor Xa.

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Year:  2004        PMID： 18007442      PMCID： PMC6147283          DOI： 10.3390/90600427

Source DB:  PubMed          Journal:  Molecules        ISSN： 1420-3049            Impact factor:   4.411


  8 in total

Review 1.  Strategies for the inhibition of serine proteases.

Authors:  B Walker; J F Lynas
Journal:  Cell Mol Life Sci       Date:  2001-04       Impact factor: 9.261

Review 2.  The coagulation cascade: initiation, maintenance, and regulation.

Authors:  E W Davie; K Fujikawa; W Kisiel
Journal:  Biochemistry       Date:  1991-10-29       Impact factor: 3.162

3.  Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay.

Authors:  A Anastasi; C G Knight; A J Barrett
Journal:  Biochem J       Date:  1993-03-01       Impact factor: 3.857

4.  Determination of the P1', P2' and P3' subsite-specificity of factor Xa.

Authors:  Justin P Ludeman; Robert N Pike; Karen M Bromfield; Peter J Duggan; Julia Cianci; Bernard Le Bonniec; James C Whisstock; Stephen P Bottomley
Journal:  Int J Biochem Cell Biol       Date:  2003-02       Impact factor: 5.085

5.  The role of Glu(192) in the allosteric control of the S(2)' and S(3)' subsites of thrombin.

Authors:  P E Marque; R Spuntarelli; L Juliano; M Aiach; B F Le Bonniec
Journal:  J Biol Chem       Date:  2000-01-14       Impact factor: 5.157

6.  Intramolecularly quenched fluorogenic substrates for hydrolytic enzymes.

Authors:  A Yaron; A Carmel; E Katchalski-Katzir
Journal:  Anal Biochem       Date:  1979-05       Impact factor: 3.365

7.  Characterization of the P2' and P3' specificities of thrombin using fluorescence-quenched substrates and mapping of the subsites by mutagenesis.

Authors:  B F Le Bonniec; T Myles; T Johnson; C G Knight; C Tapparelli; S R Stone
Journal:  Biochemistry       Date:  1996-06-04       Impact factor: 3.162

8.  The action of factor Xa on peptide p-nitroanilide substrates: substrate selectivity and examination of hydrolysis with different reaction conditions.

Authors:  R Lottenberg; J A Hall; E Pautler; A Zupan; U Christensen; C M Jackson
Journal:  Biochim Biophys Acta       Date:  1986-12-12
  8 in total

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