Literature DB >> 18007048

Purification, crystallization and preliminary X-ray diffraction study of human ribosomal protein L10 core domain.

Mitsuhiro Nishimura1, Tatsuya Kaminishi, Masahito Kawazoe, Mikako Shirouzu, Chie Takemoto, Shigeyuki Yokoyama, Akiko Tanaka, Sumio Sugano, Takuya Yoshida, Tadayasu Ohkubo, Yuji Kobayashi.   

Abstract

Eukaryotic ribosomal protein L10 is an essential component of the large ribosomal subunit, which organizes the architecture of the aminoacyl-tRNA binding site. The human L10 protein is also called the QM protein and consists of 214 amino-acid residues. For crystallization, the L10 core domain (L10CD, Phe34-Glu182) was recombinantly expressed in Escherichia coli and purified to homogeneity. A hexagonal crystal of L10CD was obtained by the sitting-drop vapour-diffusion method. The L10CD crystal diffracted to 2.5 A resolution and belongs to space group P3(1)21 or P3(2)21.

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Year:  2007        PMID： 18007048      PMCID： PMC2339757          DOI： 10.1107/S1744309107048142

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  24 in total

1.  Qsr1p, a 60S ribosomal subunit protein, is required for joining of 40S and 60S subunits.

Authors:  D P Eisinger; F A Dick; B L Trumpower
Journal:  Mol Cell Biol       Date:  1997-09       Impact factor: 4.272

2.  SQT1, which encodes an essential WD domain protein of Saccharomyces cerevisiae, suppresses dominant-negative mutations of the ribosomal protein gene QSR1.

Authors:  D P Eisinger; F A Dick; E Denke; B L Trumpower
Journal:  Mol Cell Biol       Date:  1997-09       Impact factor: 4.272

3.  Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.

Authors:  C R Woese; O Kandler; M L Wheelis
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

4.  QM is a novel zinc-binding transcription regulatory protein: its binding to c-Jun is regulated by zinc ions and phosphorylation by protein kinase C.

Authors:  H Inada; J Mukai; S Matsushima; T Tanaka
Journal:  Biochem Biophys Res Commun       Date:  1997-01-13       Impact factor: 3.575

5.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

6.  QSR1, an essential yeast gene with a genetic relationship to a subunit of the mitochondrial cytochrome bc1 complex, codes for a 60 S ribosomal subunit protein.

Authors:  F A Dick; S Karamanou; B L Trumpower
Journal:  J Biol Chem       Date:  1997-05-16       Impact factor: 5.157

7.  Extreme evolutionary conservation of QM, a novel c-Jun associated transcription factor.

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Journal:  Hum Mol Genet       Date:  1994-05       Impact factor: 6.150

8.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.

Authors:  J D Thompson; D G Higgins; T J Gibson
Journal:  Nucleic Acids Res       Date:  1994-11-11       Impact factor: 16.971

9.  The yeast growth control gene GRC5 is highly homologous to the mammalian putative tumor suppressor gene QM.

Authors:  H T Koller; T Klade; A Ellinger; M Breitenbach
Journal:  Yeast       Date:  1996-01       Impact factor: 3.239

10.  QSR1, an essential yeast gene with a genetic relationship to a subunit of the mitochondrial cytochrome bc1 complex, is homologous to a gene implicated in eukaryotic cell differentiation.

Authors:  T Tron; M Yang; F A Dick; M E Schmitt; B L Trumpower
Journal:  J Biol Chem       Date:  1995-04-28       Impact factor: 5.157
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  1 in total

1.  Cooperative down-regulation of ribosomal protein L10 and NF-κB signaling pathway is responsible for the anti-proliferative effects by DMAPT in pancreatic cancer cells.

Authors:  Chen Shi; Yang Wang; Yuna Guo; Yijun Chen; Nan Liu
Journal:  Oncotarget       Date:  2017-05-23
  1 in total

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