Literature DB >> 18007047

Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase.

Hiroyuki Morita1, Shin Kondo, Ryohei Kato, Kiyofumi Wanibuchi, Hiroshi Noguchi, Shigetoshi Sugio, Ikuro Abe, Toshiyuki Kohno.   

Abstract

Octaketide synthase (OKS) from Aloe arborescens is a plant-specific type III polyketide synthase that produces SEK4 and SEK4b from eight molecules of malonyl-CoA. Recombinant OKS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group I422, with unit-cell parameters a = b = 110.2, c = 281.4 A, alpha = beta = gamma = 90.0 degrees . Diffraction data were collected to 2.6 A resolution using synchrotron radiation at BL24XU of SPring-8.

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Year:  2007        PMID： 18007047      PMCID： PMC2339743          DOI： 10.1107/S1744309107047069

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  6 in total

1.  Crystallography & NMR system: A new software suite for macromolecular structure determination.

Authors:  A T Brünger; P D Adams; G M Clore; W L DeLano; P Gros; R W Grosse-Kunstleve; J S Jiang; J Kuszewski; M Nilges; N S Pannu; R J Read; L M Rice; T Simonson; G L Warren
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1998-09-01

2.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

3.  Engineered biosynthesis of plant polyketides: chain length control in an octaketide-producing plant type III polyketide synthase.

Authors:  Ikuro Abe; Satoshi Oguro; Yoriko Utsumi; Yukie Sano; Hiroshi Noguchi
Journal:  J Am Chem Soc       Date:  2005-09-14       Impact factor: 15.419

4.  Engineered biosynthesis of novel polyketides: evidence for temporal, but not regiospecific, control of cyclization of an aromatic polyketide precursor.

Authors:  H Fu; D A Hopwood; C Khosla
Journal:  Chem Biol       Date:  1994-12

5.  A plant type III polyketide synthase that produces pentaketide chromone.

Authors:  Ikuro Abe; Yoriko Utsumi; Satoshi Oguro; Hiroyuki Morita; Yukie Sano; Hiroshi Noguchi
Journal:  J Am Chem Soc       Date:  2005-02-09       Impact factor: 15.419

6.  Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens.

Authors:  Hiroyuki Morita; Shin Kondo; Satoshi Oguro; Hiroshi Noguchi; Shigetoshi Sugio; Ikuro Abe; Toshiyuki Kohno
Journal:  Chem Biol       Date:  2007-04
  6 in total
  2 in total

Review 1.  How structural subtleties lead to molecular diversity for the type III polyketide synthases.

Authors:  Hiroyuki Morita; Chin Piow Wong; Ikuro Abe
Journal:  J Biol Chem       Date:  2019-08-30       Impact factor: 5.157

2.  Desorption Electrospray Ionization (DESI) Mass Spectrometric Imaging of the Distribution of Rohitukine in the Seedling of Dysoxylum binectariferum Hook. F.

Authors:  Patel Mohana Kumara; Amitava Srimany; Suganya Arunan; Gudasalamani Ravikanth; Ramanan Uma Shaanker; Thalappil Pradeep
Journal:  PLoS One       Date:  2016-06-30       Impact factor: 3.240
  2 in total

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