BACKGROUND: Transplant recipients develop antibodies against a wide array of HLA specificities, and not only against the antigens to which they have been exposed. The aim of the present study was to establish if HLA-unrelated immune stimulation is capable of triggering the production of HLA antibodies. METHODS: We determined the presence of HLA antibodies in 20 healthy adults before and after the administration of an immune stimulus (hepatitis B vaccine plus tuberculin skin test). HLA antibodies were assessed with a flow-cytometry based system. RESULTS: At baseline, HBsAg antibodies were detected in protective titers in 75% of the subjects; HLA antibodies were negative in all but one. One week after the antigenic stimulus, HBsAg antibody titers increased significantly, without any detectable changes in HLA antibodies. Surprisingly, HLA antibodies developed in 8 participants one month after the application of the stimulus. One additional subject developed HLA antibodies one month later. Therefore 9/20 subjects became PRA positive during the observation period. Antibody specificities were identified by single antigen assay. The alloantibody response elicited by the immune stimulus was not consistent with memory cell stimulation. CONCLUSIONS: The findings of this study demonstrate that a non-HLA antigenic stimulus is capable of eliciting the development of HLA antibodies in healthy adults.
BACKGROUND: Transplant recipients develop antibodies against a wide array of HLA specificities, and not only against the antigens to which they have been exposed. The aim of the present study was to establish if HLA-unrelated immune stimulation is capable of triggering the production of HLA antibodies. METHODS: We determined the presence of HLA antibodies in 20 healthy adults before and after the administration of an immune stimulus (hepatitis B vaccine plus tuberculin skin test). HLA antibodies were assessed with a flow-cytometry based system. RESULTS: At baseline, HBsAg antibodies were detected in protective titers in 75% of the subjects; HLA antibodies were negative in all but one. One week after the antigenic stimulus, HBsAg antibody titers increased significantly, without any detectable changes in HLA antibodies. Surprisingly, HLA antibodies developed in 8 participants one month after the application of the stimulus. One additional subject developed HLA antibodies one month later. Therefore 9/20 subjects became PRA positive during the observation period. Antibody specificities were identified by single antigen assay. The alloantibody response elicited by the immune stimulus was not consistent with memory cell stimulation. CONCLUSIONS: The findings of this study demonstrate that a non-HLA antigenic stimulus is capable of eliciting the development of HLA antibodies in healthy adults.
Authors: Robert S Nickel; Jeanne E Hendrickson; Marianne M Yee; Robert A Bray; Howard M Gebel; Leslie S Kean; David B Miklos; John T Horan Journal: Br J Haematol Date: 2015-04-19 Impact factor: 6.998
Authors: Julie M Yabu; Matthew W Anderson; Deborah Kim; Brian D Bradbury; Calvin D Lou; Jeffrey Petersen; Jerome Rossert; Glenn M Chertow; Dolly B Tyan Journal: Nephrol Dial Transplant Date: 2013-09-05 Impact factor: 5.992
Authors: Darrell J Triulzi; Steven Kleinman; Ram M Kakaiya; Michael P Busch; Philip J Norris; Whitney R Steele; Simone A Glynn; Christopher D Hillyer; Patricia Carey; Jerome L Gottschall; Edward L Murphy; Jorge A Rios; Paul M Ness; David J Wright; Danielle Carrick; George B Schreiber Journal: Transfusion Date: 2009-05-18 Impact factor: 3.157
Authors: Dapeng Li; Simon Brackenridge; Lucy C Walters; Olivia Swanson; Karl Harlos; Daniel Rozbesky; Derek W Cain; Kevin Wiehe; Richard M Scearce; Maggie Barr; Zekun Mu; Robert Parks; Max Quastel; Robert J Edwards; Yunfei Wang; Wes Rountree; Kevin O Saunders; Guido Ferrari; Persephone Borrow; E Yvonne Jones; S Munir Alam; Mihai L Azoitei; Geraldine M Gillespie; Andrew J McMichael; Barton F Haynes Journal: Commun Biol Date: 2022-03-28