Literature DB >> 1800525

A simple method for isolation of DNA from formalin-fixed paraffin-embedded samples for PCR.

P Kallio1, S Syrjänen, A Tervahauta, K Syrjänen.   

Abstract

A simple method of processing formalin-fixed, paraffin-embedded tissue sections for DNA amplification by polymerase chain reaction (PCR) is described. In this procedure, deparaffinized sections are readily subjected to DNA isolation simply by boiling and the released DNA can be directly employed for PCR. The method allows analysis of single-copy genes or viral sequences at least up to 300 base pairs long in one working day. This method is particularly useful in analysing retrospective materials when the simplicity and low cost of the assay are preferable. Furthermore, the simplicity of the procedure reduces the risk of contamination.

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Year:  1991        PMID： 1800525     DOI： 10.1016/0166-0934(91)90083-c

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  7 in total

1.  Known HPV types have no association with keratoacanthomas.

Authors:  S Lu; S L Syrjänen; V K Havu; S Syrjänen
Journal:  Arch Dermatol Res       Date:  1996-03       Impact factor: 3.017

2.  Viruses and interstitial cystitis: adenovirus genomes cannot be demonstrated in urinary bladder biopsies.

Authors:  V Hukkanen; M Haarala; M Nurmi; P Klemi; P Kiilholma
Journal:  Urol Res       Date:  1996

3.  Detection and identification of mycobacteria in formalin-fixed, paraffin-embedded tissues by nested PCR and restriction enzyme analysis.

Authors:  C R Bascuñana; K Belák
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

4.  No evidence of human papillomavirus DNA in actinic keratosis.

Authors:  S Lu; K Syrjänen; V K Havu
Journal:  Arch Dermatol Res       Date:  1995       Impact factor: 3.017

5.  TP53 gene mutations in gastric carcinoma detected by polymerase chain reaction/single-strand conformation polymorphism analysis of archival material.

Authors:  E D'Andrea; R Baffa; C Menin; M Montagna; M Rugge; L Chieco-Bianchi
Journal:  J Cancer Res Clin Oncol       Date:  1995       Impact factor: 4.553

6.  Use of neuropathological tissue for molecular genetic studies: parameters affecting DNA extraction and polymerase chain reaction.

Authors:  S Kösel; M B Graeber
Journal:  Acta Neuropathol       Date:  1994       Impact factor: 17.088

Review 7.  Applications of DNA amplification techniques in veterinary diagnostics.

Authors:  M Pfeffer; M Wiedmann; C A Batt
Journal:  Vet Res Commun       Date:  1995       Impact factor: 2.459
  7 in total

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