Sakhila K Banu1, JeHoon Lee, Anna Starzinski-Powitz, Joe A Arosh. 1. Department of Integrative Biosciences, Reproductive Endocrinology and Cell Signaling Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, USA.
Abstract
OBJECTIVE: To determine whether immortalized endometriotic cells can be used as potential models to unravel pathophysiology/pathogenesis of endometriosis in humans. DESIGN: In vitro study. Human immortalized endometriotic epithelial and stromal cells. SETTING: College of Veterinary Medicine and Biomedical Sciences, Texas A&M University. PATIENT(S): Not applicable. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Gene expression pattern and prostaglandin E(2) production and activity of matrix metalloproteinase 2 and matrix metalloproteinase 9. RESULT(S): Several genes associated with estrogen and progesterone biosynthesis and signaling, cell cycle regulation, extracellular matrix degradation, angiogenesis, cell growth and survival, cytokine production, and prostaglandin E(2) biosynthesis, transport, and signaling were expressed in human immortalized endometriotic cells. These endometriotic epithelial and stromal cells exhibited migrating and invading potential, produced a high quantity of prostaglandin E(2), and showed higher matrix metalloproteinase 2 and matrix metalloproteinase 9 activity compared to normal endometrial, epithelial, and stromal cells. CONCLUSION(S): Human immortalized endometriotic epithelial and stromal cells could be used as ideal models to study the molecular and cellular aspects of endometriosis in humans.
OBJECTIVE: To determine whether immortalized endometriotic cells can be used as potential models to unravel pathophysiology/pathogenesis of endometriosis in humans. DESIGN: In vitro study. Human immortalized endometriotic epithelial and stromal cells. SETTING: College of Veterinary Medicine and Biomedical Sciences, Texas A&M University. PATIENT(S): Not applicable. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Gene expression pattern and prostaglandin E(2) production and activity of matrix metalloproteinase 2 and matrix metalloproteinase 9. RESULT(S): Several genes associated with estrogen and progesterone biosynthesis and signaling, cell cycle regulation, extracellular matrix degradation, angiogenesis, cell growth and survival, cytokine production, and prostaglandin E(2) biosynthesis, transport, and signaling were expressed in human immortalized endometriotic cells. These endometriotic epithelial and stromal cells exhibited migrating and invading potential, produced a high quantity of prostaglandin E(2), and showed higher matrix metalloproteinase 2 and matrix metalloproteinase 9 activity compared to normal endometrial, epithelial, and stromal cells. CONCLUSION(S): Human immortalized endometriotic epithelial and stromal cells could be used as ideal models to study the molecular and cellular aspects of endometriosis in humans.
Authors: Kasra Khalaj; Jessica E Miller; Harshavardhan Lingegowda; Asgerally T Fazleabas; Steven L Young; Bruce A Lessey; Madhuri Koti; Chandrakant Tayade Journal: JCI Insight Date: 2019-09-19
Authors: N R Joshi; R W Su; G V R Chandramouli; S K Khoo; J W Jeong; S L Young; B A Lessey; A T Fazleabas Journal: Hum Reprod Date: 2015-09-14 Impact factor: 6.918
Authors: JeHoon Lee; Sakhila K Banu; Robert C Burghardt; Anna Starzinski-Powitz; Joe A Arosh Journal: Biol Reprod Date: 2013-03-28 Impact factor: 4.285
Authors: Chia-Hung Chen; Miles A Miller; Aniruddh Sarkar; Michael T Beste; Keith B Isaacson; Douglas A Lauffenburger; Linda G Griffith; Jongyoon Han Journal: J Am Chem Soc Date: 2012-11-29 Impact factor: 15.419