| Literature DB >> 17999917 |
Yasuo Takamune1, Tetsuro Ikebe, Osamu Nagano, Masanori Shinohara.
Abstract
The involvement of a disintegrin and metalloprotease domain (ADAM)-17 on the cancer invasion was investigated in oral squamous cell carcinoma (OSCC) cells. TNFalpha induced the conversion from the proform of ADAM-17 to its mature form time-dependently. TNFalpha also cleaved CD44 to its small fragments, as observed by a Western blot analysis. The transfection of ADAM-17 siRNA partially suppressed the expression of ADAM-17 as well as the cleavage of CD44. On the other hand, TNFalpha activated a transcription factor NF-kappaB in OSCC cells, while NBD peptide, an NF-kappaB inhibitor, inhibited the ADAM-17 maturation, thus suggesting that NF-kappaB is involved in ADAM-17 maturation. Moreover, an in vitro invasion assay revealed that both ADAM-17 siRNA and NBD peptides strongly suppressed the TNFalpha-induced invasion of OSCC cells through the matrix. In conclusion, ADAM-17 plays an important role in cancer invasion probably through CD44 cleavage.Entities:
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Year: 2007 PMID： 17999917 DOI： 10.1016/j.bbrc.2007.11.010
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575