BACKGROUND: This study focuses on the cytokine genes expression after brain-death, ischemia-reperfusion injury, and during allograft rejection. METHODS: A total of 49 needle core biopsies from kidney transplant recipients, performed before and during transplantation procedures were studied. The first biopsy was taken during procurement of the organ, the second after cold ischemia, and the third after approximately 30 min of reperfusion. We also assessed 34 allograft biopsies obtained during acute rejection. Tubular and glomerular expression of interferon (IFN)-gamma, transforming growth factor (TGF)-beta1, platelet-desired growth factor-B (PDGF-B), interleukin (IL)-2, IL-6, IL-10 mRNA was analyzed with reverse-transcription polymerase chain reaction (RT-PCR) in situ technique, which allows to detect a few copies of the target gene without destruction of the tissue architecture. RESULTS: Compared with normal kidney tissue from living donor, high gene expression of IFN-gamma, TGF-beta1, PDGF-B, IL-2, IL-6, and IL-10 was detected in all procurement specimens. After reperfusion gene expressions of IL-2, IL-6, and IL-10 were significantly upregulated in renal tubules compared to biopsies taken after cold ischemia. The gene expression of IFN-gamma, TGF-beta1, and PDGF-B remained stable after organ procurement, during cold ischemia, and after reperfusion. Gene expression of IFN-gamma, IL-2, IL-6, IL-10, and PDGF-B in procurement biopsies, as well as in those taken after cold ischemia and reperfusion, were significantly higher than during the period of acute rejection. CONCLUSION: The data presented herein strongly point out the importance of the immunological and morphological injury that occurs before and during transplantation. The increase of inflammatory response after brain death is important for further stimulation of the immune response and long-term kidney survival.
BACKGROUND: This study focuses on the cytokine genes expression after brain-death, ischemia-reperfusion injury, and during allograft rejection. METHODS: A total of 49 needle core biopsies from kidney transplant recipients, performed before and during transplantation procedures were studied. The first biopsy was taken during procurement of the organ, the second after cold ischemia, and the third after approximately 30 min of reperfusion. We also assessed 34 allograft biopsies obtained during acute rejection. Tubular and glomerular expression of interferon (IFN)-gamma, transforming growth factor (TGF)-beta1, platelet-desired growth factor-B (PDGF-B), interleukin (IL)-2, IL-6, IL-10 mRNA was analyzed with reverse-transcription polymerase chain reaction (RT-PCR) in situ technique, which allows to detect a few copies of the target gene without destruction of the tissue architecture. RESULTS: Compared with normal kidney tissue from living donor, high gene expression of IFN-gamma, TGF-beta1, PDGF-B, IL-2, IL-6, and IL-10 was detected in all procurement specimens. After reperfusion gene expressions of IL-2, IL-6, and IL-10 were significantly upregulated in renal tubules compared to biopsies taken after cold ischemia. The gene expression of IFN-gamma, TGF-beta1, and PDGF-B remained stable after organ procurement, during cold ischemia, and after reperfusion. Gene expression of IFN-gamma, IL-2, IL-6, IL-10, and PDGF-B in procurement biopsies, as well as in those taken after cold ischemia and reperfusion, were significantly higher than during the period of acute rejection. CONCLUSION: The data presented herein strongly point out the importance of the immunological and morphological injury that occurs before and during transplantation. The increase of inflammatory response after brain death is important for further stimulation of the immune response and long-term kidney survival.
Authors: A J Meltzer; G R Veillette; A Aoyama; K M Kim; M E Cochrane; J C Wain; J C Madsen; D H Sachs; B R Rosengard; J S Allan Journal: Am J Transplant Date: 2012-02-02 Impact factor: 8.086
Authors: Jennie Lin; Xuan Zhang; Chenyi Xue; Hanrui Zhang; Michael G S Shashaty; Sager J Gosai; Nuala Meyer; Alison Grazioli; Christine Hinkle; Jennifer Caughey; Wenjun Li; Katalin Susztak; Brian D Gregory; Mingyao Li; Muredach P Reilly Journal: Am J Physiol Renal Physiol Date: 2015-09-23
Authors: Dorota Kamińska; Katarzyna Kościelska-Kasprzak; Paweł Chudoba; Agnieszka Hałoń; Oktawia Mazanowska; Agnieszka Gomółkiewicz; Piotr Dzięgiel; Dominika Drulis-Fajdasz; Marta Myszka; Agnieszka Lepiesza; Wojciech Polak; Maria Boratyńska; Marian Klinger Journal: Sci Rep Date: 2016-11-03 Impact factor: 4.379
Authors: Rebecca H Vaughan; Jean-Claude Kresse; Louise K Farmer; Marie L Thézénas; Benedikt M Kessler; Jan H N Lindeman; Edward J Sharples; Gavin I Welsh; Rikke Nørregaard; Rutger J Ploeg; Maria Kaisar Journal: Am J Transplant Date: 2022-02-04 Impact factor: 9.369